{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:26:30Z","timestamp":1760549190680},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T00:00:00Z","timestamp":1334016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoporosis is a reduction in skeletal mass due to an imbalance between bone formation and bone resorption. Therefore, the identification of specific stimulators of bone formation is of therapeutic significance in the treatment of osteoporosis. Salicylideneamino\u20102\u2010thiophenol (Sal\u20102) consists of two benzene rings, has been reported to possess antioxidant activity, and is an effective remedy for fever and rheumatic diseases. However, until now the effects of osteoblastic bone formation by Sal\u20102 were unknown. In this study, we investigated the effects of Sal\u20102 on osteogenic differentiation of multipotent bone marrow stromal stem cells by alizarin red S staining for osteogenic differentiation, RT\u2010PCR and western blot for alkaline phosphatase (ALP) activity and signaling pathways, FACS analysis and immunofluorescence staining for CD44 and CD51 expression, calcium assays, and immunofluorescence staining for signaling pathways. We found that Sal\u20102 enhanced the osteogenic differentiation of multipotent bone marrow stromal stem cells. Sal\u20102 treatment induced the expression and activity of ALP, and enhanced the levels of CD44 and CD51 expression as well as Ca<jats:sup>2+<\/jats:sup> content, in multipotent bone marrow stromal stem cells. Moreover, we found that Sal\u20102\u2010induced osteogenic differentiation and expression of osteogenesis\u2010related molecules involve the activation of the MAPK and nuclear factor\u2010\u03baB pathways. Our findings provide insight into both the mechanism and effects of Sal\u20102 on osteogenic differentiation and demonstrate that Sal\u20102 may be a beneficial adjuvant in stimulating bone formation in osteoporotic diseases. J. Cell. Biochem. 113: 1833\u20131841, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.24052","type":"journal-article","created":{"date-parts":[[2012,1,6]],"date-time":"2012-01-06T13:52:25Z","timestamp":1325857945000},"page":"1833-1841","source":"Crossref","is-referenced-by-count":11,"title":["Salicylideneamino\u20102\u2010thiophenol enhances osteogenic differentiation through the activation of MAPK pathways in multipotent bone marrow stem cell"],"prefix":"10.1002","volume":"113","author":[{"given":"Hyung Keun","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Soon","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun Sik","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji 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