{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:47:06Z","timestamp":1698212826399},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have used differential hybridization to isolate and characterize two novel cDNAs expressed in chondrocytes and some osteoblastic cells. A rat osteosarcoma ROS17\/2.8 cDNA library was screened and cDNA clones hybridizing strongly to radiolabeled porcine calvaria cDNA but weakly to a control radiolabeled cDNA were isolated. Two clones were obtained\u2014p.6.1 and p.10.15. A radiolabeled probe of p10.15 was shown to hybridize specifically to a 2.3 Kb message RNA from a chondrogenic clonal cell population from rat calvaria\u2010RCJ 3.1C5.18, and the mRNA was downregulated by 1,25 (OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>, which inhibits chondrogenesis in these cells. The other clone, p6.1, was found to hybridize to a 0.95 Kb message that is expressed in rat liver, kidney, lung, muscle, and brain, but not expressed in spleen and expressed only in low levels in thymus.<\/jats:p>","DOI":"10.1002\/jcb.240540303","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T21:33:40Z","timestamp":1117316020000},"page":"273-280","source":"Crossref","is-referenced-by-count":2,"title":["Isolation of cDNA clones from an osteosarcoma\u2010ROS17\/2.8 library by differential hybridization"],"prefix":"10.1002","volume":"54","author":[{"given":"Mary M. Y.","family":"Waye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent K. 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