{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:23:21Z","timestamp":1773300201355,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3642,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mitochondrial response to the effect of a hydroxylase (PH) inhibitor was tested in marrow stromal cells during stimulation of osteoprogenitor cell (OPC) differentiation. The rationale for this was to explore pathways of regulatory interactions between procollagen synthesis and mitochondrial respiration that could be linked to the commitment of OPCs to mineralization. Stimulated OPCs exposed to the PH inhibitor cis\u2010hydroxyproline (cis\u2010HP), compared to the noninhibiting isomer trans\u2010HP, for 11 days showed a dose\u2010dependent decrease in cell proliferation, the surviving cells showed increased alkaline phosphatase activity. Trans\u2010HP did not influence the cis\u2010HP effect on ALP and on proliferation arrest. Short time exposures, 2\u20133 days, to cis\u2010HP at different periods suggested that Days 0\u20133 and 3\u20135 were critical for the commitment to Day 21 mineralization of OPCs. On Days 0\u20133 cells were most sensitive to cis\u2010HP, since on Day 11, 8 days after removal of cis\u2010HP, they were too scarce to be counted by the staining method. However, the presence of 5.0 mM cis\u2010HP in the cultures during Days 3\u20135 has induced on Day 21 close to 24\u2010fold more mineralization\/cell than controls, compared to the trans\u2010HP effect, which was only close to 3\u2010fold. The presence of cis\u2010HP in the cultures on Days 0\u20133 has augmented the mitochondrial Day 3 retention of rhodamine 123 (Rho) in the stromal cells, relative to controls, compared to the presence of trans\u2010HP. However, the presence of cis\u2010HP during Days 3\u20135 or 3\u20136 resulted in lower Day 5 Rho retention, relative to controls, which was not significantly different from the retention that resulted from trans\u2010HP. Since Rho retention is related to the final result of aerobic respiration level, these results are interpreted as a cis\u2010HP inhibitory effect on procollagen peptidyl\u2010proline hydroxylation, which may in turn release oxygen surpluses, to be available for mitochondrial consumption. The fall in Rho retention responses to cis\u2010HP between Days 0\u20133 and 3\u20135 is suggesting either abrupt decrease in proline hydroxylation or poor mitochondrial sensitivity to oxygen in Day 3\u20135 cultures.<\/jats:p>","DOI":"10.1002\/jcb.240540311","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T21:33:40Z","timestamp":1117316020000},"page":"354-364","source":"Crossref","is-referenced-by-count":11,"title":["Marrow stromal cell commitment to mineralization under the effect of a prolyl hydroxylase inhibitor"],"prefix":"10.1002","volume":"54","author":[{"given":"Benjamin 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