{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T16:40:28Z","timestamp":1756572028757},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3550,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transcriptional regulation of gene expression in vivo in bone, associated with normal development or skeletal disorders, to date, has not been studied. We report the successful isolation of nuclei that are transcriptionally active from normal and osteopetrotic rat bone. Transcription rates of cell growth and bone\u2010related genes (including histone H4, <jats:italic>c<\/jats:italic>\u2010<jats:italic>fos<\/jats:italic>, <jats:italic>c<\/jats:italic>\u2010<jats:italic>jun<\/jats:italic>, TGF\u03b21, \u03b2<jats:sub>2<\/jats:sub> macroglobulin, collagen, fibronectin, osteocalcin, osteopontin, and tartrate resistent acid phosphatase) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals. Additionally, nuclei isolated from intact bone yield promoter binding factors. Bone nuclei, which transcribe faithfully and contain the normal complement of nuclear protein factors, offer a powerful approach for investigating in vivo gene regulation in skeletal development and pathology. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.240550205","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T21:37:26Z","timestamp":1117316246000},"page":"182-189","source":"Crossref","is-referenced-by-count":9,"title":["Transcriptionally active nuclei isolated from intact bone reflect modified levels of gene expression in skeletal development and pathology"],"prefix":"10.1002","volume":"55","author":[{"given":"V.","family":"Shalhoub","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Bortell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"S. C.","family":"Marks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Stein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. B.","family":"Lian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. S.","family":"Stein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/332166a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041430203"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02556874"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650070816"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38511-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.5.1475"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00320a049"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/311433a0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-2-1107"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0021-9258(17)31295-4","article-title":"Molecular cloning of the type 5, iron\u2010containing, tartrate\u2010resistant acid phosphatase from human placenta","volume":"264","author":"Ketcham CM","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041460306"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-2-955"},{"key":"e_1_2_1_15_1","first-page":"S82","article-title":"Concentrations of osteocalcin and phosphoprotein as a function of mineral content and age in cortical bone","volume":"34","author":"Lian JB","year":"1982","journal-title":"Calcif Tissue Int"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.4.1143"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650020411"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.5.1701"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001380204"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90073-4"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02411278"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.11.4687"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650020212"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.23.8819"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.6.3183"},{"key":"e_1_2_1_26_1","first-page":"433","volume-title":"Biology and Physiology of the Osteoclast","author":"Osier LK","year":"1992"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041430304"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041390214"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02557826"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90175-7"},{"key":"e_1_2_1_31_1","doi-asserted-by":"crossref","first-page":"9847","DOI":"10.1016\/S0021-9258(18)92897-8","article-title":"Gene expression during skeletal development in three osteopetrotic rat mutations","volume":"266","author":"Shalhoub V","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240500411"},{"key":"e_1_2_1_33_1","unstructured":"ShalhoubV BettencourtB JacksonME MacKayCA GlimcherMJ MarksSCJr SteinGS LianJB(in press): Abnormalities of phosphoprotein gene expression in three osteopetrotic rat mutations: Elevated mRNA transcripts protein synthesis and accumulation in bone of mutant animals.J Cell Physiol."},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240500410"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1992.263.4.E724"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.11.5367"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(87)80250-4"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240550205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240550205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T23:47:21Z","timestamp":1698104841000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240550205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,6]]}},"alternative-id":["10.1002\/jcb.240550205"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240550205","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6]]}}}