{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T20:47:55Z","timestamp":1760906875480},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3397,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1994,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have described in the first paper the coupling betweencytosolic Gi\u03b1 and cytosolic PLC activity in a cell free preparation. In order to establish the functional significance of the cytosolic Gi\u03b1 coupled soluble PLC, we examined the effects of Dex, NaF, and trifluopeerizine (TEP) on concanavalin A(Con A)\u2010induced PI\u2010turnover in intact slenocytes and, in parallel, on soluble PLC activity in cytosol preparations. Vytosolic PLC activity was measured with [<jats:sup>3<\/jats:sup>H]PIP and [<jats:sup>3<\/jats:sup>H]PIP<jats:sub>2<\/jats:sub> as substrates. (1) The con A\u2010induced increase (2\u20134 fold) in Pl\u2010turnover in intact splenocytes was paralleled by an 1.2\u20135\u2010fold increase in soluble PLC activity in vitro. Con A administration also increased cytosolic Gi\u03b1 immunoreactivity 3\u20136\u2010fold as expected if cytosolic Gi\u03b1 was coupled to soluble PLC activation. (2) DEX (10<jats:sup>\u22127<\/jats:sup> M), administered 6 h prior to Con A administration inbited the Con A\u2010induced increase in Pl\u2010turnover in intact splenocytes. This was paralleled by DEX inhibition of the Con A\u2010induced increase in soluble PLC activity measured in vitro and cytosolic Gi\u03b1 imunoreactivity. (3) We have demonstrated in the first paper that NaF and TEP inhibited soluble PLC activity. Here we show that NaF and TFP inhibited the Con A\u2010induced increase in PI\u2010turnover extending the similarities between soluble PLC activity and Con A\u2010 Stimulated PLC Activity in intact splenocytes. (4) In order to examine Whether or not the Con A\u2010induced PLC activity and Con A\u2010stimulated PLC activity in intact splenocytes. (4) In order to examine Whether or not the Con A\u2010induced PLC was similar to PLC\u03b3, we measured PI\u2010turnover induced by Con A or BaVO<jats:sub>3<\/jats:sub> in combination with DEX and PMA. Whereas the Con A\u2010induced PI\u2010turnover was significantly inhibited (40\u201360%) by DEX, the NaVO<jats:sub>3<\/jats:sub> \u2010induced PI\u2010turnover was not affected by DEX. The Con A\u2010induced PI\u2010turnover was not affected by PMA (50nM), But the NaVO<jats:sub>3<\/jats:sub>\u2010induced Pi\u2010turnover was increased over 2\u2010fold PMA (50nM), suggesting that the Con A\u2010induced PLC in intact splenocytes is different from NaVO<jats:sub>3<\/jats:sub>\u2010induced PLC. Based on these results a model for the sequential activation of substrate\u2010specific PLCs in splenocyte by mitogen is presented.<\/jats:p>","DOI":"10.1002\/jcb.240560317","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T21:50:14Z","timestamp":1117317014000},"page":"409-417","source":"Crossref","is-referenced-by-count":1,"title":["Cytosolic phospholipase C activity: II. Relationship to concanavalin A\u2010induced phosphatidylinositol\u2010turnover in splenocytes"],"prefix":"10.1002","volume":"56","author":[{"given":"Thomas","family":"Akompong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Spencer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce S.","family":"McEwen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.5.1963"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240560316"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60756-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"23069","DOI":"10.1016\/S0021-9258(18)50057-0","article-title":"Activation of cytosolic phosphoinositide phospholipase C by G\u2010protein \u03b2\u03b3 subunits","volume":"267","author":"Blank JL","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"25451","DOI":"10.1016\/S0021-9258(19)74062-9","article-title":"\u03b2\u03b3\u2010subunit activation of G\u2010protein\u2010regulated phospholipase C","volume":"267","author":"Boyer JL","year":"1992","journal-title":"J. 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