{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:21:37Z","timestamp":1759962097867},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3216,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1995,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Brunner's glandsl are located in the submucosa of the proximal duodenum and are unique to mammalian species. The North American opossum <jats:italic>(Didelphis virginiana)<\/jats:italic> is generally regarded as a prototype marsupial that closely resembles fossil didelphids which can be placed at the beginning of mammalian evolution. The current investigation provided an opportunity for the analysis of secretory products from these glands in a species thought to be more closely related to earlier evolutionary forms. Extracts of Brunner's glands were subjected to SDS\u2010PAGE and Western Blotting. The results indicate the presence of two high molecular weight PAS\u2010positive glycoprotein bands. In addition to these two PAS\u2010positive bands, several other glycoprotein bands were detected in the high molecular weight range that bind several lectins which typically recognize O\u2010linked carbohydrates indicative of mucus type glycoproteins. The same lectins bind to glandular structures in tissue sections. Comparison of lectin binding sites with the pyloric glands of the stomach and duodenal goblet cells indicates that brunner's glands carbohydrate residues resemble those of the pyloric glands more closely than those of the duodenal goblet cells. The low cell turnover rate in brunner's glands is in contrast to the rapid turnover rate of goble cell precursors in the duodenal crypts. The mucus composition and the cell turnover rate correlate well with embryological data and suggest that Brunner's glands of <jats:italic>Didelphis<\/jats:italic> evolved from an epithelium more closely associated closely associated with the stomach than that of the duodenum as the topography of the gland would suggest. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.240580108","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T21:53:16Z","timestamp":1117317196000},"page":"56-64","source":"Crossref","is-referenced-by-count":7,"title":["Molecular anatomy of an endodermal gland: Investigations on mucus glycoproteins and cell turnover in Brunner's glands of didelphis verginiana using lectins and PCNA immunoreactivity"],"prefix":"10.1002","volume":"58","author":[{"given":"U.","family":"Schumacher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"Krause","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091670408"},{"key":"e_1_2_1_3_1","first-page":"67","volume-title":"Ultrastructure of the Digestive Tract","author":"Krause WJ","year":"1987"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-77287-0_1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001260303"},{"key":"e_1_2_1_6_1","first-page":"11","article-title":"The effect of surgical aboation of Brunner's glands on the proximal duldenal mucosa","volume":"166","author":"Krause WJ","year":"1988","journal-title":"Anat Anz"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(93)90066-Z"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/BF00570291","article-title":"Histochemistry of mucosubstances in Brunner's\u2010gland cells and duodenal goblet cells of two New World monkey species(Saimiri sciureus and Saguinus fuscicollis)","volume":"88","author":"Posel P","year":"1998","journal-title":"Histochemistry"},{"key":"e_1_2_1_9_1","first-page":"128","volume-title":"Man and the vertebrates","author":"Romer AS","year":"1971"},{"key":"e_1_2_1_10_1","first-page":"11","article-title":"Recovery of Proteins from the brocho\u2010alveolar lavage fluid. Proposal for a standardisation","volume":"30","author":"Schumacher U","year":"1992","journal-title":"Eur J Clin Chem Clin Biochem"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.290110311"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/42.1.7903329"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(84)90047-X"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-9254-9_23"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1042\/bj2030779","article-title":"Isolation and Partial characterization of rat duodenal\u2010gland(Bruner's gland) mucus glycoprotein","volume":"203","author":"Smits HL","year":"1982","journal-title":"Biochem J"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409239209082559"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/BF00570295","article-title":"Structural, histochemical and biochemical observations on horse milk\u2010fat\u2010globule membranes and casein micelles","volume":"88","author":"Welsh U","year":"1998","journal-title":"Histochemistry"},{"key":"e_1_2_1_18_1","first-page":"723","volume-title":"Lectins Biology, Biochemistry, Clinical Biochemistry","author":"Wu AM","year":"1998"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240580108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240580108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T22:41:49Z","timestamp":1698273709000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240580108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,5]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,5]]}},"alternative-id":["10.1002\/jcb.240580108"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240580108","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,5]]}}}