{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:34:39Z","timestamp":1760204079519},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3032,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Despite its recognition as the most prevalent HIV associated cancer, speculation still abounds regarding the pathogenesis of AIDS\u2010related Kaposi's sarcoma (AIDS\u2010KS). However, it has been established that both cytokines, e.g. IL\u20106, and HIV\u2010associated products, e.g., Tat, are integral in AIDS\u2010KS cellular proliferation. Further, both experimental and clinical evidence is accumulating to link reactive oxygen intermediates (ROI) with both cytokine induction (primarily via nuclear factor\u2010\u03baB [NF\u2010\u03baB] dependent routes) as well as the subsequent cytokine, tumor necrosis factor \u03b1 (TNF\u03b1) stimulation of HIV replication. Features of AIDS\u2010KS patients, such as retention of phagocytes, presence of sustained immunostimulation, and a frequent history of KS lesions arising at traumatized sites, make oxidant stress a viable clinical factor in AIDS\u2010KS development. Time course nucleotide profile analyses show that AIDS\u2010KS cells have an inherent, statistically significant, biochemical deficit, even prior to oxidant stress, due to (1) a more glycolytic bioenergetic profile, resulting in lower levels of high energy phosphates (impairing capacity for glutathione [GSH] synthesis and DNA repair); (2) lower levels of NADPH (compromising the activities of GSSG reductase and peroxidase function of catalase); and (3) reduced levels of GSH (impeding both GSH peroxidase and GSH\u2010S\u2010transferases). Following exposure to physiologically relevant levels of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> only the human microvascular endothelial cells (a putative AIDS\u2010KS progenitor cell) responded with bioenergetic adaptations that reflected co\u2010ordination of energy generating and cytoprotective pathways, e.g., retention of the cellular energy charge, increased NAD<jats:sup>+<\/jats:sup>, and an accentuation of the ATP, NADPH, and total adenine nucleotide differences relative to AIDS\u2010KS cells. Also, some of the AIDS\u2010KS strains retained intracellular GSSG subsequent to oxidant challenge, inviting the formation of deleterious protein mixed disulfides. While the results of our study address some AIDS\u2010KS issues, they also raise an etiological question, i.e., Does the inability to tolerate oxidant stress arise in conjunction with AIDS\u2010KS neoplastic development, or is it pre\u2010existing in the population at risk? Regardless, use of antioxidant therapy (low risk\/potentially high benefit) in both the \u201cat risk\u201d population as well as in those individuals with active disease may prove a useful preventative and\/or treatment modality. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.240590304","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T22:13:42Z","timestamp":1117318422000},"page":"317-328","source":"Crossref","is-referenced-by-count":16,"title":["Cultured AIDS\u2010related kaposi's sarcoma (AIDS\u2010KS) cells demonstrate impaired bioenergetic adaptation to oxidant challenge: Implication for oxidant stress in AIDS\u2010KS pathogenesis"],"prefix":"10.1002","volume":"59","author":[{"given":"S. R.","family":"Mallery","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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