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In this investigation, the 17\u03b2\u2010hydroxysteroid oxidoreductase activity of cell lines derived from two ovarian tumors (OVCAR\u20103, CAOV\u20103) and an epidermoid tumor of the vulva (A431) was assayed under conditions which differentiate between 17\u03b2\u2010hydroxysteriod oxidoreductase type 1, a cytosolic isoform highly specific for estradiol, and type 2, a membrane bound isoform reactive with both estradiol and testosterone. On the basic of estradiol\/testosterone activity ratios, all three cell lines appear to have type 2\u2010like activity, with the specific activity of A431 markedly greater than that of the other cell lines. Estradiol, progesterone, or EGE, alone or in combination, were without effect on the enzymatic activity of OVCAR\u20103 cells. EGE decreased the activity of CAOV\u20103 cells slightly. In contrast, EGE stimulated A431 17\u03b2\u2010hydroxysteriod oxidoreductase activity 7\u20138\u2010fold over a 5\u2010day exposure. Estradiol or progesterone, singly or in combination, also did not effect the enzymatic activity of A431 cells. However, progesterone inhibited the increase in activity seen in the presence of EGE. With EGE, estradiol, and progesterone together, the increase in enzymatic activity was comparable to that with EGE alone. The effects of estradiol and progesterone appear to result from steroid actions following binding of EGE to low\u2010affinity receptors on A431 cells. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcb.240590402","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T22:14:01Z","timestamp":1117318441000},"page":"409-417","source":"Crossref","is-referenced-by-count":11,"title":["Properties and regulation of 17\u03b2\u2010hydroxysteroid oxidoreductase of OVCAR\u20103, CAOV\u20103, and A431 cells: Effects of epidermal growth factor, estradiol, and progesterone"],"prefix":"10.1002","volume":"59","author":[{"given":"Charles H.","family":"Blomquist","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin S.","family":"Leung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youyang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia M.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1180149"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(11)90598-5"},{"key":"e_1_2_1_4_1","first-page":"241","article-title":"Molecular and biologic factors in the pathogenesis of ovarian cancer","volume":"39","author":"Berek JS","year":"1994","journal-title":"J Reprod Med"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(78)90460-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(84)90204-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(85)90828-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(93)90209-F"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(94)90009-4"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.132.1.179"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1390715"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.2.565"},{"key":"e_1_2_1_13_1","first-page":"2895","article-title":"Estradiol 17\u03b2\u2010hydroxysteroid dehydrogenase, a marker of breast cancer hormone dependency","volume":"45","author":"Fournior S","journal-title":"Cancer Res"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0594-34"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/293305a0","article-title":"Incresed phosphotyrosine content and inhibition of proliferation in EGF\u2010treated A431 cells","volume":"293","author":"Gill GN","year":"1981","journal-title":"Nature"},{"key":"e_1_2_1_16_1","first-page":"146","article-title":"The molecular biology of RU486. 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