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We used novel, pH\u2010responsive nanoparticles to deliver functionally active siRNAs into cultures of salivary gland cells. The nanoparticle molecules are comprised of cationic micelles that electrostatically interact with the siRNA, protecting it from nuclease attack, and also include pH\u2010responsive endosomolytic constituents that promote release of the siRNA into the target cell cytoplasm. Transfection controls with Cy3\u2010tagged siRNA\/nanoparticle complexes showed efficiently internalized siRNAs in more than 70% of the submandibular gland cells. We found that introduction of siRNAs specifically targeting the Pkc\u03b4 or Bax genes significantly blocked the induction of these pro\u2010apoptotic proteins that normally occurs after radiation in cultured salivary gland cells. Furthermore, the level of cell death from subsequent radiation, as measured by caspase\u20103, TUNEL, and mitochondrial disruption assays, was significantly decreased. Thus, we have successfully demonstrated that the siRNA\/nanoparticle\u2010mediated knock down of pro\u2010apoptotic genes can prevent radiation\u2010induced damage in submandibular gland primary cell cultures. J. Cell. Biochem. 113: 1955\u20131965, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.24064","type":"journal-article","created":{"date-parts":[[2012,1,17]],"date-time":"2012-01-17T18:58:56Z","timestamp":1326826736000},"page":"1955-1965","source":"Crossref","is-referenced-by-count":29,"title":["Pro\u2010apoptotic gene knockdown mediated by nanocomplexed siRNA reduces radiation damage in primary salivary gland cultures"],"prefix":"10.1002","volume":"113","author":[{"given":"Szilvia","family":"Arany","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Hernady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle 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