{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T03:04:29Z","timestamp":1778036669188,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,10,9]],"date-time":"2012-10-09T00:00:00Z","timestamp":1349740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>NELL\u20101 (Nel\u2010like molecule\u20101) is a secreted osteogenic growth factor first identified in human craniosynostosis (CS) patients. NELL\u20101 protein has been observed to promote bone and cartilage differentiation and to suppress adipogenesis in both in vitro and in vivo models. Despite these findings, the cell surface receptors of NELL\u20101 have remained unknown. In this study, we observed for the first time that NELL\u20101 promotes cell adherence in multiple cell lines, including ST2, C3H10T1\/2, M2\u201010B4, ATDC5, and MC3T3 cells. Additionally, we found that NELL\u20101 binds to extracellular Integrin\u03b21 and induces cell focal adhesion. By utilizing siRNA methods, we determined that NELL\u20101 cell surface binding and enhanced cell attachment were dependent on Integrin\u03b21 expression. Finally, we observed that pre\u2010coating of culture dishes or PLGA (polylactic\u2010<jats:italic>co<\/jats:italic>\u2010glycolic acid) scaffold with NELL\u20101 resulted in a significant increase in both cell attachment and osteogenic differentiation. Our results identify for the first time a cell surface target of NELL\u20101, Integrin\u03b21, and elucidate new functions of NELL\u20101 in promoting cell adherence and osteogenic differentiation. J. Cell. Biochem. 113: 3620\u20133628, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.24253","type":"journal-article","created":{"date-parts":[[2012,7,14]],"date-time":"2012-07-14T03:06:06Z","timestamp":1342235166000},"page":"3620-3628","source":"Crossref","is-referenced-by-count":46,"title":["NELL\u20101 promotes cell adhesion and differentiation via integrin\u03b21"],"prefix":"10.1002","volume":"113","author":[{"given":"Jia","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron W.","family":"James","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonguk","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiho","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James B.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin S.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyong M.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoaki","family":"Niimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun'ichi","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang","family":"Ting","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia","family":"Soo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.051210"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300270"},{"key":"e_1_2_5_4_1","unstructured":"AllenASS.2002.A novel technique of computer\u2010assisted image analysis to quantify molecular stress in cetaceans.http:\/\/www.mediacy.com\/pdfs\/021209cetaceans.pdf"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.12.006"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.314"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0141"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-011-9440-4"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0714"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-413-1_14"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.11.014"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.113878"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.06.111"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007120"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100109-104104"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0384"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0550"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200109098"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-010-0046-y"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.296.5573.1652"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161206777947740"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32909"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.1.C132"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2957"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.30034"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.045112"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0486"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0142"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2005.09.017"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.1.80"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106377109"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1616229"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15375"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0705"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.267"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/SCS.0b013e318240c8c4"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510376401"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.06.024"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.24253","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.24253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T16:29:29Z","timestamp":1696868969000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.24253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,9]]},"references-count":37,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1002\/jcb.24253"],"URL":"https:\/\/doi.org\/10.1002\/jcb.24253","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,9]]}}}