{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T07:11:29Z","timestamp":1696921889599},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T00:00:00Z","timestamp":1361145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>p53 protein, the central molecule of the apoptosis pathway, is mutated in 50% of the human cancers. Of late, p53 homologues have been identified from different invertebrates including <jats:italic>Drosophila melanogaster<\/jats:italic>, <jats:italic>Caenorhabditis elegans<\/jats:italic>, Squid, and Clams. We report the identification of a p53\u2010like protein in <jats:italic>Spodoptera frugiperda<\/jats:italic> (<jats:italic>Sf<\/jats:italic>9) insect cells, which is activated during oxidative stress, caused by exposure to UV\u2010B or H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, and binds to p53 consensus DNA binding motifs as well as other p53 cognate motifs. <jats:italic>Sf<\/jats:italic>9 p53 motif\u2010binding protein is similar to murine and Drosophila p53 in terms of molecular size, which is around 50\u201360\u2009kDa, as evident from UV cross\u2010linking, and displays DNA binding characteristics similar to both insect and vertebrate p53 as seen from electrophoretic mobility shift assays. The N\u2010terminal sequencing of the purified <jats:italic>Sf<\/jats:italic>9 p53 motif\u2010binding protein reveals extensive homology to the pro\u2010apoptotic FK\u2010506 binding protein (FKBP\u201046), earlier identified in <jats:italic>Sf<\/jats:italic>9 cells as a factor which interacts with murine casein kinase. FKBP, an evolutionarily conserved protein of mammalian origin functions as a pro\u2010apoptotic factor. Identification of FKBP\u201046 as a novel p53 motif\u2010binding protein in insect cells adds a new facet to our understanding of the mechanisms of apoptosis under oxidative stress in the absence of a typical p53 homologue. J. Cell. Biochem. 114: 899\u2013907, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.24429","type":"journal-article","created":{"date-parts":[[2012,10,23]],"date-time":"2012-10-23T17:17:49Z","timestamp":1351012669000},"page":"899-907","source":"Crossref","is-referenced-by-count":0,"title":["<i>Spodoptera frugiperda<\/i> FKBP\u201046 is a consensus p53 motif binding protein"],"prefix":"10.1002","volume":"114","author":[{"given":"Krishnaveni","family":"Mohareer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudhir","family":"Sahdev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed 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