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Indinavir and nelfinavir induced a significant decrease in tyrosine phosphorylation of the insulin receptor \u03b2\u2010subunit. Indinavir caused a significant increase in the phosphorylation of insulin receptor substrate\u20101 (IRS\u20101) on serine 307 (S307) in both CHO\u2010IR cells and 3T3\u2010L1 adipocytes. Nelfinavir also inhibited phosphorylation of Map\/ERK kinase without affecting insulin\u2010stimulated Akt phosphorylation. Concomitantly, levels of protein tyrosine phosphatase 1B (PTP1B), suppressor of cytokines signaling\u20101 and \u20103 (SOCS\u20101 and \u20103), Src homology 2B (SH2B) and adapter protein with a pleckstrin homology domain and an SH2 domain (APS) were not altered significantly. When CHO\u2010IR cells were pre\u2010treated with sodium salicylate (NaSal), the effects of indinavir on tyrosine phosphorylation of the IR \u03b2\u2010subunit and phosphorylation of IRS\u20101 at S307 were abrogated. These data suggest a potential role for the NF\u03baB pathway in insulin resistance induced by HPIs. J. Cell. Biochem. 114: 1729\u20131737, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcb.24513","type":"journal-article","created":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T13:35:58Z","timestamp":1360071358000},"page":"1729-1737","source":"Crossref","is-referenced-by-count":12,"title":["Indinavir and nelfinavir inhibit proximal insulin receptor signaling and salicylate abrogates inhibition: Potential role of the NFkappa B pathway"],"prefix":"10.1002","volume":"114","author":[{"given":"Wan Iryani W.","family":"Ismail","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy A.","family":"King","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khawar","family":"Anwar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tahir 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