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However, their adverse effects such as nephrotoxicity and auditory ototoxicity, as well as some toxic effects directed to pigmented tissues, complicate the use of these agents. This study was undertaken to investigate the effect of aminoglycoside antibiotic\u2014kanamycin on viability, melanogenesis and antioxidant enzymes activity in cultured human normal melanocytes (HEMa\u2010LP). It has been demonstrated that kanamycin induces concentration\u2010dependent loss in melanocytes viability. The value of EC<jats:sub>50<\/jats:sub> was found to be \u223c6.0\u2009mM. Kanamycin suppressed melanin biosynthesis: antibiotic was shown to inhibit cellular tyrosinase activity and to reduce melanin content in normal human melanocytes. Significant changes in the cellular antioxidant enzymes: SOD, CAT and GPx were stated in melanocytes exposed to kanamycin. Moreover, it was observed that kanamycin caused depletion of antioxidant defense sytem. It is concluded that the inhibitory effect of kanamycin on melanogenesis and not sufficient antioxidant defense mechanism in melanocytes in vitro may explain the potential mechanisms of undesirable side effects of this drug directed to pigmented tissues in vivo. J. Cell. Biochem. 114: 2746\u20132752, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.24623","type":"journal-article","created":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T17:31:10Z","timestamp":1372267870000},"page":"2746-2752","source":"Crossref","is-referenced-by-count":16,"title":["Impact of kanamycin on melanogenesis and antioxidant enzymes activity in melanocytes\u2014an in vitro study"],"prefix":"10.1002","volume":"114","author":[{"given":"Dorota","family":"Wrze\u015bniok","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Department of Pharmaceutical Chemistry Medical University of Silesia Jagiello\u0144ska 4, PL 41\u2010200 Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Otr\u0119ba","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Pharmaceutical Chemistry Medical University of Silesia Jagiello\u0144ska 4, PL 41\u2010200 Sosnowiec 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