{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:14:41Z","timestamp":1788534881680,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,12,13]],"date-time":"2013-12-13T00:00:00Z","timestamp":1386892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Research Foundation (NRF), Proof-of-Concept grant:","award":["R-174-000-139-281"],"award-info":[{"award-number":["R-174-000-139-281"]}]},{"name":"Academic Research Fund, National University of Singapore (AcRF-NUS)","award":["R-174-000-129-112"],"award-info":[{"award-number":["R-174-000-129-112"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Wound healing is a major problem in diabetic patients and current treatments have met with limited success. We evaluated the treatment of excisional and diabetic wounds using a stem cell isolated from the human umbilical cord Wharton's jelly (hWJSC) that shares unique properties with embryonic and adult mesenchymal stem cells. hWJSCs are non\u2010controversial, available in abundance, hypo\u2010immunogenic, non\u2010tumorigenic, differentiate into keratinocytes, and secrete important molecules for tissue repair. When human skin fibroblasts (CCD) in conventional scratch\u2010wound assays were exposed to hWJSC\u2010conditioned medium (hWJSC\u2010CM) the fibroblasts at the wound edges migrated and completely covered the spaces by day 2 compared to controls. The number of invaded cells, cell viability, total collagen, elastin, and fibronectin levels were significantly greater in the hWJSC\u2010CM treatment arm compared to controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). When a single application of green fluorescent protein (GFP)\u2010labeled hWJSCs (GFP\u2010hWJSCs) or hWJSC\u2010CM was administered to full\u2010thickness murine excisional and diabetic wounds, healing rates were significantly greater compared to controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Wound biopsies collected at various time points showed the presence of green GFP\u2010labeled hWJSCs, positive human keratinocyte markers (cytokeratin, involucrin, filaggrin) and expression of ICAM\u20101, TIMP\u20101, and VEGF\u2010A. On histology, the GFP\u2010hWJSCs and hWJSC\u2010CM treated wounds showed reepithelialization, increased vascularity and cellular density and increased sebaceous gland and hair follicle numbers compared to controls. hWJSCs showed increased expression of several miRNAs associated with wound healing compared to CCDs. Our studies demonstrated that hWJSCs enhance healing of excisional and diabetic wounds via differentiation into keratinocytes and release of important molecules. J. Cell. Biochem. 115: 290\u2013302, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.24661","type":"journal-article","created":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T12:30:48Z","timestamp":1377779448000},"page":"290-302","source":"Crossref","is-referenced-by-count":74,"title":["Human Wharton's Jelly Stem Cells and Its Conditioned Medium Enhance Healing of Excisional and Diabetic Wounds"],"prefix":"10.1002","volume":"115","author":[{"given":"Chui\u2010Yee","family":"Fong","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology Yong Loo Lin School of Medicine, National University Health System, National University of Singapore Singapore 119228 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberley","family":"Tam","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Yong Loo Lin School of Medicine, National University Health System, National University of 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