{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T15:55:22Z","timestamp":1783698922119,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,12,13]],"date-time":"2013-12-13T00:00:00Z","timestamp":1386892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81271160"],"award-info":[{"award-number":["81271160"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Scientific Research Foundation of Guangdong Province, China","award":["A2012106"],"award-info":[{"award-number":["A2012106"]}]},{"name":"Nature Science Foundation of Guangdong Province, China","award":["S2012010010393"],"award-info":[{"award-number":["S2012010010393"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>To investigate whether crosstalk between <jats:italic>RUNX2<\/jats:italic> and miRNAs is involved in tooth eruption regulated by dental follicle cells (DFCs) and the possible molecular mechanism. Blood samples and embedded dental follicles were collected from patients with cleidocranial dysplasia, and <jats:italic>RUNX2<\/jats:italic> gene mutations were analyzed, then <jats:italic>RUNX2<\/jats:italic><jats:sup>+\/m<\/jats:sup> DFCs were isolated and identified. The characteristics of <jats:italic>RUNX2<\/jats:italic><jats:sup>+\/m<\/jats:sup> DFCs were analyzed. The differential expression of miRNAs was detected between the <jats:italic>RUNX2<\/jats:italic><jats:sup>+\/m<\/jats:sup> DFCs and <jats:italic>RUNX2<\/jats:italic><jats:sup>+\/+<\/jats:sup> DFCs by microarray, and target genes were predicted by miRGen. miR\u2010146a was chosen for further investigation, and its effects in DFCs were analyzed by transfecting its mimics and inhibitors, and expression of genes involved in tooth eruption were detected. A novel insertion mutation (c.309_310insTG) of <jats:italic>RUNX2<\/jats:italic> gene was identified which had an effect on the characteristics of DFCs. Compared with the <jats:italic>RUNX2<\/jats:italic><jats:sup>+\/+<\/jats:sup> DFCs, there were 69 microRNAs more than twofold up\u2010regulated and 54 microRNAs more than twofold down\u2010regulated in the <jats:italic>RUNX2<\/jats:italic><jats:sup>+\/m<\/jats:sup> DFCs. Among these, miR\u2010146a decreased significantly in <jats:italic>RUNX<\/jats:italic>2<jats:sup>+\/m<\/jats:sup> DFCs, and expression of <jats:italic>RUNX2<\/jats:italic>, <jats:italic>CSF\u20101<\/jats:italic>, <jats:italic>EGFR<\/jats:italic>, and <jats:italic>OPG<\/jats:italic> was significantly altered when miR\u2010146a was overexpressed or inhibited. <jats:italic>RUNX2<\/jats:italic> gene mutation contributes to the characteristic change of DFCs, and the crosstalk between <jats:italic>RUNX2<\/jats:italic> gene and miRNAs may be one of the key regulatory mechanisms of differentiation of DFCs. J. Cell. Biochem. 115: 340\u2013348, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.24668","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T13:29:32Z","timestamp":1378992572000},"page":"340-348","source":"Crossref","is-referenced-by-count":24,"title":["Effect and Possible Mechanism of Network Between Micro<scp>RNA<\/scp>s and <scp><i>RUNX<\/i><\/scp><i>2<\/i> Gene on Human Dental Follicle Cells"],"prefix":"10.1002","volume":"115","author":[{"given":"Pei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Periodontology Guangdong Provincial Stomatological Hospital, Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dixin","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Periodontology Guangdong Provincial Stomatological Hospital, Southern Medical University Guangzhou 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