{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:24:27Z","timestamp":1790630667538,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T00:00:00Z","timestamp":1402963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004334","name":"Merck & Co., Inc.","doi-asserted-by":"publisher","award":["40643"],"award-info":[{"award-number":["40643"]}],"id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Cathepsin K (CatK) is a lysosomal cysteine protease necessary for bone resorption by osteoclasts (OCs), which originate from myeloid hematopoietic precursors. CatK\u2010deficient (CatK<jats:sup>\u2212\/\u2212<\/jats:sup>) mice show osteopetrosis due to defective resorption by OCs, which are increased in number in these mice. We investigated whether genetic ablation of CatK altered the number of hematopoietic stem cells (HSCs) and OC precursor cells (OCPs) using two mouse models: CatK<jats:sup>\u2212\/\u2212<\/jats:sup> mice and a knock\u2010in mouse model in which the CatK gene (<jats:italic>ctsk<\/jats:italic>) is replaced by <jats:italic>cre<\/jats:italic> recombinase. We found that CatK deletion in mice significantly increased the number of HSCs in the spleen and decreased their number in bone marrow. In contrast, the number of early OCPs was unchanged in the bone marrow. However, the number of committed CD11b<jats:sup>+<\/jats:sup> OCPs was increased in the bone marrow of CatK<jats:sup>\u2212\/\u2212<\/jats:sup> compared to wild\u2010type (WT) mice. In addition, the percentage but not the number of OCPs was decreased in the spleen of CatK<jats:sup>\u2212\/\u2212<\/jats:sup> mice relative to WT. To understand whether increased commitment to OC lineage in CatK<jats:sup>\u2212\/\u2212<\/jats:sup> mice is influenced by the bone marrow microenvironment, CatK<jats:sup>Cre\/+<\/jats:sup> or CatK<jats:sup>Cre\/Cre<\/jats:sup> red fluorescently labeled OCPs were injected into WT mice, which were also subjected to a mid\u2010diaphyseal femoral fracture. The number of OCs derived from the intravenously injected CatK<jats:sup>Cre\/Cre<\/jats:sup> OCPs was lower in the fracture callus compared to mice injected with CatK<jats:sup>+\/Cre<\/jats:sup> OCPs. Hence, in addition to its other effects, the absence of CatK in OCP limits their ability to engraft in a repairing fracture callus compared to WT OCP. J. Cell. Biochem. 115: 1449\u20131457, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.24801","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T05:50:55Z","timestamp":1393912255000},"page":"1449-1457","source":"Crossref","is-referenced-by-count":17,"title":["Altered Hematopoietic Stem Cell and Osteoclast Precursor Frequency in Cathepsin K Null Mice"],"prefix":"10.1002","volume":"115","author":[{"given":"Christian","family":"Jacome\u2010Galarza","sequence":"first","affiliation":[{"name":"New England Musculoskeletal Institute University of Connecticut Health Center Farmington Connecticut 06030"},{"name":"Department of Medicine University of Connecticut Health Center Farmington Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Yu","family":"Soung","sequence":"additional","affiliation":[{"name":"New England Musculoskeletal Institute University of Connecticut Health Center Farmington Connecticut 06030"},{"name":"Department of Orthopaedics University of Connecticut Health Center Farmington Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naga Suresh","family":"Adapala","sequence":"additional","affiliation":[{"name":"New England Musculoskeletal Institute University of Connecticut Health Center Farmington Connecticut 06030"},{"name":"Department of Orthopaedics University of Connecticut Health Center Farmington Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen","family":"Pickarski","sequence":"additional","affiliation":[{"name":"Merck Sharp &amp; Dohme Research Laboratories West Point Philadelphia 19486"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Sanjay","sequence":"additional","affiliation":[{"name":"New England Musculoskeletal Institute University of Connecticut Health Center Farmington Connecticut 06030"},{"name":"Department of Orthopaedics University 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