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CK2 downregulation is known to impact mitochondrial apoptotic circuitry but the underlying mechanism(s) remain unclear. Utilizing prostate cancer cell lines subjected to CK2\u2010specific inhibitors which cause loss of cell viability, we have found that CK2 inhibition in cells causes rapid early decrease in mitochondrial membrane potential (\u0394\u03c8<jats:sub>m<\/jats:sub>). Cells treated with the CK2 inhibitors TBB (4,5,6,7\u2010tetrabromobenzotriazole) or TBCA (tetrabromocinnamic acid) demonstrate changes in \u0394\u03c8<jats:sub>m<\/jats:sub> which become apparent within 2\u2009h, that is, significantly prior to evidence of activation of other mitochondrial apoptotic signals whose temporal expression ensues subsequent to loss of \u0394\u03c8<jats:sub>m<\/jats:sub>. Further, we have demonstrated the presence of CK2 in purified mitochondria and it appears that the effect on \u0394\u03c8<jats:sub>m<\/jats:sub> evoked by inhibition of CK2 may involve mitochondrial localized CK2. Results also suggest that alterations in Ca<jats:sup>2+<\/jats:sup> signaling may be involved in the CK2 mediated regulation of \u0394\u03c8<jats:sub>m<\/jats:sub> and mitochondrial permeability. Thus, we propose that a key mechanism of CK2 impact on mitochondrial apoptotic circuitry and cell death involves early loss of \u0394\u03c8<jats:sub>m<\/jats:sub> which may be a primary trigger for apoptotic signaling and cell death resulting from CK2 inhibition. J. Cell. Biochem. 115: 2103\u20132115, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.24887","type":"journal-article","created":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T04:37:34Z","timestamp":1405571854000},"page":"2103-2115","source":"Crossref","is-referenced-by-count":32,"title":["Protein Kinase CK2 Inhibition Induces Cell Death via Early Impact on Mitochondrial Function"],"prefix":"10.1002","volume":"115","author":[{"given":"Fatima","family":"Qaiser","sequence":"first","affiliation":[{"name":"Cellular and Molecular Biochemistry Research Laboratory (151) Minneapolis Veterans Affairs Health Care System Minneapolis Minnesota 55417"},{"name":"Department of Laboratory Medicine and Pathology University of Minnesota School of Medicine Minneapolis MN 55455"},{"name":"Department of Biochemistry and Molecular Biology, Army Medical College National University of Sciences and Technology Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janeen H.","family":"Trembley","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biochemistry Research Laboratory (151) Minneapolis Veterans Affairs Health Care System Minneapolis Minnesota 55417"},{"name":"Department of Laboratory Medicine and Pathology University of Minnesota School of Medicine Minneapolis MN 55455"},{"name":"Masonic Cancer Center University of Minnesota Minneapolis Minnesota 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betsy T.","family":"Kren","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biochemistry Research Laboratory (151) Minneapolis Veterans Affairs Health Care System Minneapolis Minnesota 55417"},{"name":"Department of Medicine University of Minnesota Minneapolis Minnesota 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Jiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Biochemistry Research Laboratory (151) Minneapolis Veterans Affairs Health Care System Minneapolis Minnesota 55417"},{"name":"Department of Laboratory Medicine and Pathology University of Minnesota School of Medicine Minneapolis MN 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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