{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T21:26:14Z","timestamp":1775510774545,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T00:00:00Z","timestamp":1431388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FONDECYT","award":["1130045"],"award-info":[{"award-number":["1130045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Postmenopausal osteoporosis is characterized by decreased bone quality and mineral density. Mesenchymal stem cells (MSCs) found in the bone marrow, are pluripotent cells able to differentiate into several phenotypes, including osteoblasts and adipocytes. In osteoporosis, MSCs' commitment and differentiation into osteoblast\/adipocyte is unbalanced, favoring adipocyte formation. The osteo and adipogenic processes are modulated by the bone morphogenetic protein\u20102 (BMP\u20102). This cytokine regulates the expression of transcription factors PPAR\u03b3 and Runx 2, but its action on cells under adipogenic conditions is poorly understood. In this work we studied BMP\u20102 signaling in MSCs obtained from bone marrow of control or osteoporotic volunteer postmenopausal women. MSCs were cultured under basal, adipogenic (AD) or AD plus BMP\u20102 conditions. The protein content of PPAR\u03b3, p\u2010PPAR\u03b3, Runx2, bone morphogenetic receptor IA (BMPR IA), phosphorylated Smad\u20101\/5\/8 (p\u2010Smad) and Smad 4 were determined by specific western blots. mRNA level for BMPRs was determined by PCR and cell localization of p\u2010Smad\u20101\/5\/8 were detected by immunocytochemistry. Control MSCs showed a differential response to both AD and AD plus BMP\u20102 treatments: BMP\u20102 exerted an anti\u2010adipogenic effect increasing both transcription factors analyzed. Moreover, p\u2010Smads\u20101\/5\/8 were detected in nuclei after short term BMP\u20102 treatment. Osteoporotic MSCs showed no response to exogenous added BMP\u20102, as shown by p\u2010PPAR\u03b3\/PPAR\u03b3 ratio and Runx2 levels, although BMPR\u2010IA level was significantly higher in osteoporotic than in control MSCs. In addition, staining for p\u2010Smad\u20101\/5\/8 in o\u2010MSCs was observed around nuclei at all experimental conditions. Taken together results demonstrate failure of BMP\u20102 signaling in osteoporotic MSCs. J. Cell. Biochem. 116: 1267\u20131277, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25082","type":"journal-article","created":{"date-parts":[[2015,1,31]],"date-time":"2015-01-31T21:16:00Z","timestamp":1422738960000},"page":"1267-1277","source":"Crossref","is-referenced-by-count":40,"title":["Osteoporosis\u2010associated alteration in the signalling status of BMP\u20102 in human MSCs under adipogenic conditions"],"prefix":"10.1002","volume":"116","author":[{"given":"Oscar","family":"Donoso","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular INTA Universidad de Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Mar\u00eda","family":"Pino","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular INTA Universidad de Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germ\u00e1n","family":"Seitz","sequence":"additional","affiliation":[{"name":"Hospital S\u00f3tero del R\u00edo Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson","family":"Osses","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Pontificia Universidad Cat\u00f3lica de Valpara\u00edso"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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