{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:03:39Z","timestamp":1785211419251,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Estradiol (E2) is a major determinant of gender\u2010based differences in the development of hepatic fibrosis. MicroRNAs (miRNAs) are endogenous 19\u201025 nucleotide, noncoding, single\u2010stranded RNAs that regulate gene expression by blocking the translation or decreasing the stability of mRNAs and play an important role in liver fibrosis. The mechanisms underlying the regulation of miRNAs by E2 remain largely unknown. In this study, miR\u201019b levels were higher and were associated with lower GRB2 mRNA and protein levels in female rats more than in male rats. We also showed that miR\u201019b levels were down\u2010regulated, were associated with the up\u2010regulation of GRB2 mRNA and protein levels in PS (porcine serum\u2010induced hepatic fibrosis) versus NS (normal control) groups and were up\u2010regulated when associated with the down\u2010regulation of GRB2 mRNA and protein levels in PS\u2009+\u2009E2 versus PS and in aHSC\u2009+\u2009E2 (estradiol treated aHSC) versus aHSC groups. MiR\u201019b expression inhibited cell proliferation in aHSCs, and also down\u2010regulated GRB2 protein expression. The overexpression of miR\u201019b inhibited cell growth and suppressed COL1A1 protein levels by decreasing the levels of GRB2. However, the forced expression of GRB2 partly rescued the effect of miR\u201019b in the cells, attenuated cell proliferation, and suppressed the GRB2 protein level by up\u2010regulating the levels of GRB2. Taken together, these findings will shed light on the role of miR\u201019b in regulating aHSC proliferation via the miR\u201019b\/GRB2 axis. This newly identified miR\u201019b\/GRB2 interaction provided novel insights into the suppressive effect of E2 on HSC proliferation and might facilitate the development of therapies targeting hepatic fibrosis. J. Cell. Biochem. 116: 2455\u20132464, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25116","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T22:32:45Z","timestamp":1423002765000},"page":"2455-2464","source":"Crossref","is-referenced-by-count":25,"title":["MicroRNA\u201019b Reduces Hepatic Stellate Cell Proliferation by Targeting GRB2 in Hepatic Fibrosis Models In Vivo and In Vitro as Part of the Inhibitory Effect of Estradiol"],"prefix":"10.1002","volume":"116","author":[{"given":"Shanfei","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Infectious Disease Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Xiangya Hospital Central South University Changsha Hunan 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