{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:17:31Z","timestamp":1740104251657,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea(NRF)","doi-asserted-by":"crossref","award":["2009-0094027"],"award-info":[{"award-number":["2009-0094027"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Tissue microenvironment adjusts biological properties of different cells by modulating signaling pathways and cell to cell interactions. This study showed that epithelial\u2013mesenchymal transition (EMT)\/ mesenchymal\u2013epithelial transition (MET) can be modulated by altering culture conditions. HPV E6\/E7\u2010transfected immortalized oral keratinocytes (IHOK) cultured in different media displayed reversible EMT\/MET accompanied by changes in cell phenotype, proliferation, gene expression at transcriptional, and translational level, and migratory and invasive activities. Cholera toxin, a major supplement to culture medium, was responsible for inducing the morphological and biological changes of IHOK. Cholera toxin per se induced EMT by triggering the secretion of interleukin 6 (IL\u20106) from IHOK. We found IL\u20106 to be a central molecule that modulates the reversibility of EMT based not only on the mRNA level but also on the level of secretion. Taken together, our results demonstrate that IL\u20106, a cytokine whose transcription is activated by alterations in culture conditions, is a key molecule for regulating reversible EMT\/MET. This study will contribute to understand one way of cellular adjustment for surviving in unfamiliar conditions. J. Cell. 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