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Using microarray, real\u2010time RT\u2010PCR, radioimmunoassay analysis and western blot, we first showed that supplementation of low\u2010dose of IGF\u2010I in culture medium resulted in enlarged cell size of Lantang SCs, only Akt and S6K were up\u2010regulated at both the mRNA and protein levels among almost all of the mTOR pathway key genes, but had no effect on cell number. To elucidate the signaling mechanisms responsible for regulating cell size under low\u2010dose of IGF\u2010I treatment, we blocked Akt and S6K activity with the specific inhibitors MK2206 and PF4708671, respectively. Both inhibitors caused a decrease in cell size. In addition, MK2206 lowered the protein level of p\u2010Akt (Ser473), p\u2010S6K (Thr389), and p\u2010rpS6 (Ser235\/236), whereas PF4708671 lowered the protein level of p\u2010S6K (Thr389) and p\u2010rpS6 (Ser235\/236). However, low dose of IGF\u2010I didn't affect the protein level of p\u2010mTOR (Ser2448) and p\u2010mTOR (Ser2481). When both inhibitors were applied simultaneously, the effect was the same as that of the Akt inhibition alone. Taken together, we report for the first time that low\u2010dose of IGF\u2010I treatment increases cell size via Akt\/S6K signaling pathway. J. Cell. Biochem. 116: 2637\u20132648, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25212","type":"journal-article","created":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T12:50:12Z","timestamp":1430311812000},"page":"2637-2648","source":"Crossref","is-referenced-by-count":17,"title":["Low Dose of IGF\u2010I Increases Cell Size of Skeletal Muscle Satellite Cells Via Akt\/S6K Signaling Pathway"],"prefix":"10.1002","volume":"116","author":[{"given":"Chun\u2010qi","family":"Gao","sequence":"first","affiliation":[{"name":"College of Animal Science, South China Agricultural University\/National Engineering Research Center for Breeding Swine Industry\/Guangdong Provincial Key Laboratory of Agro\u2010Animal Genomics Guangzhou Guangdong province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhi","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University\/National 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