{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:19:17Z","timestamp":1777425557508,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research (ICMR)","doi-asserted-by":"crossref","award":["58\/9\/2009-BMS"],"award-info":[{"award-number":["58\/9\/2009-BMS"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Activation of fatty acid synthase (FASN) enzyme in the de novo lipogenic pathway has been reported in various cancers including retinoblastoma (RB), a pediatric ocular cancer. The present study investigates lipogenesis\u2010dependent survival of RB cancer cells and the associated molecular pathways in <jats:italic>FASN<\/jats:italic> silenced RB cells. The siRNA\u2010mediated FASN gene knockdown in RB cancer cells (Y79, WERI RB1) repressed FASN mRNA and protein expressions, and decreased cancer cell viability. Global gene expression microarray analysis was performed in optimized FASN siRNA transfected and untransfected RB cells. Deregulation of various downstream cell signaling pathways such as EGFR (n\u2009=\u200955 genes), TGF\u2010beta (n\u2009=\u200945 genes), cell cycle (n\u2009=\u200941 genes), MAPK (n\u2009=\u200939 genes), lipid metabolism (n\u2009=\u200923 genes), apoptosis (n\u2009=\u200921 genes), GPCR signaling (n\u2009=\u200921 genes), and oxidative phosporylation (n\u2009=\u200918 genes) were observed. The qRT\u2010PCR validation in <jats:italic>FASN<\/jats:italic> knockdown RB cells revealed up\u2010regulation of <jats:italic>ANXA1<\/jats:italic>, <jats:italic>DAPK2<\/jats:italic>, and down\u2010regulation of <jats:italic>SKP2<\/jats:italic>, <jats:italic>SREBP1c<\/jats:italic>, <jats:italic>RXRA<\/jats:italic>, <jats:italic>ACACB<\/jats:italic>, <jats:italic>FASN<\/jats:italic>, <jats:italic>HMGCR<\/jats:italic>, <jats:italic>USP2a<\/jats:italic> genes that favored the anti\u2010cancer effect of lipogenic inhibition in RB. The expression of these genes in primary RB tumor tissues were correlated with <jats:italic>FASN<\/jats:italic> expression, based on their clinico\u2010pathological features. The differential phosphorylation status of the various PI3K\/AKT pathway proteins (by western analysis) indicated that the FASN gene silencing indeed mediated apoptosis in RB cells through the PI3K\/AKT pathway. Scratch assay clearly revealed that FASN silencing reduced the invading property of RB cancer cells. Dependence of RB cancer cells on lipid metabolism for survival and progression is implicated. Thus targeting FASN is a promising strategy in RB therapy. J. Cell. Biochem. 116: 2676\u20132694, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25217","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T23:05:28Z","timestamp":1431126328000},"page":"2676-2694","source":"Crossref","is-referenced-by-count":14,"title":["Global Gene Deregulations in <i>FASN<\/i> Silenced Retinoblastoma Cancer Cells: Molecular and Clinico\u2010Pathological Correlations"],"prefix":"10.1002","volume":"116","author":[{"given":"Manoharan","family":"Sangeetha","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Birla Institute of Technology and Science (BITS) Pilani Rajasthan India"},{"name":"L and T Department of Ocular Pathology Vision Research Foundation Sankara Nethralaya Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perinkulam Ravi","family":"Deepa","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Birla 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