{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:09:19Z","timestamp":1785283759439,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T00:00:00Z","timestamp":1444262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Program Project","award":["HL-48788"],"award-info":[{"award-number":["HL-48788"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Early work in pressure overloaded (PO) myocardium shows that integrins mediate focal adhesion complex formation by recruiting the adaptor protein p130Cas (Cas) and nonreceptor tyrosine kinase c\u2010Src. To explore c\u2010Src role in Cas\u2010associated changes during PO, we used a feline right ventricular in vivo PO model and a three\u2010dimensional (3D) collagen\u2010embedded adult cardiomyocyte in vitro model that utilizes a Gly\u2010Arg\u2010Gly\u2010Asp\u2010Ser (RGD) peptide for integrin stimulation. Cas showed slow electrophoretic mobility (band\u2010shifting), recruitment to the cytoskeleton, and tyrosine phosphorylation at 165, 249, and 410 sites in both 48\u2009h PO myocardium and 1\u2009h RGD\u2010stimulated cardiomyocytes. Adenoviral mediated expression of kinase inactive (negative) c\u2010Src mutant with intact scaffold domains (KN\u2010Src) in cardiomyocytes did not block the RGD stimulated changes in Cas. Furthermore, expression of KN\u2010Src or kinase active c\u2010Src mutant with intact scaffold function (A\u2010Src) in two\u2010dimensionally (2D) cultured cardiomyocytes was sufficient to cause Cas band\u2010shifting, although tyrosine phosphorylation required A\u2010Src. These data indicate that c\u2010Src's adaptor function, but not its kinase function, is required for a serine\/threonine specific phosphorylation(s) responsible for Cas band\u2010shifting. To explore this possibility, Chinese hamster ovary cells that stably express Cas were infected with either \u03b2\u2010gal or KN\u2010Src adenoviruses and used for Cas immunoprecipitation combined with mass spectrometry analysis. In the KN\u2010Src expressing cells, Cas showed phosphorylation at the serine\u2010639 (human numbering) site. A polyclonal antibody raised against phospho\u2010serine\u2010639 detected Cas phosphorylation in 24\u201348\u2009h PO myocardium. Our studies indicate that c\u2010Src's adaptor function mediates serine\u2010639 phosphorylation of Cas during integrin activation in PO myocardium. J. Cell. Biochem. 116: 2793\u20132803, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25224","type":"journal-article","created":{"date-parts":[[2015,5,14]],"date-time":"2015-05-14T23:43:29Z","timestamp":1431647009000},"page":"2793-2803","source":"Crossref","is-referenced-by-count":3,"title":["A Kinase\u2010Independent Function of c\u2010Src Mediates p130Cas Phosphorylation at the Serine\u2010639 Site in Pressure Overloaded Myocardium"],"prefix":"10.1002","volume":"116","author":[{"given":"Arun P.","family":"Palanisamy","sequence":"first","affiliation":[{"name":"Division of Cardiology, Department of Medicine Gazes Cardiac Research Institute, Medical University of South Carolina Charleston South Carolina 29425\u20102221"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geetha","family":"Suryakumar","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine Gazes Cardiac Research Institute, Medical University of South Carolina Charleston South Carolina 29425\u20102221"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavin","family":"Panneerselvam","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine Gazes Cardiac Research Institute, Medical University of South Carolina Charleston South Carolina 29425\u20102221"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Willey","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine Gazes Cardiac Research Institute, Medical University of South Carolina Charleston South Carolina 29425\u20102221"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhandapani","family":"Kuppuswamy","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine Gazes Cardiac Research Institute, Medical University of South Carolina Charleston South Carolina 29425\u20102221"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501258200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.16.5865-5878.2000"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.120709"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1161\/01.RES.33.2.213","article-title":"1973. Mechanisms for the abnormal energetics of pressure\u2010induced hypertrophy of cat myocardium","volume":"33","author":"Cooper G.","journal-title":"Circ Res"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5762"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2509"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/1246"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2014.07.002"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E11-03-0207"},{"key":"e_1_2_6_11_1","first-page":"H1717","article-title":"Contractile function of isolated feline cardiocytes in response to viscous loading","volume":"257","author":"Kent RL","year":"1989","journal-title":"Am J Physiol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.7.4500"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006124200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2007.01.002"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2009.05.006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.389981"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00060-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1282"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(99)01714-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20031797"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(08)80044-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.22.7641-7652.2001"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200954"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1456-8"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.3.1702"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(03)00112-3"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4.184"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.8.4023"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.25224","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.25224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T13:46:45Z","timestamp":1696600005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.25224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,8]]},"references-count":27,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1002\/jcb.25224"],"URL":"https:\/\/doi.org\/10.1002\/jcb.25224","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,8]]}}}