{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:50:36Z","timestamp":1779915036958,"version":"3.53.1"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T00:00:00Z","timestamp":1444262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NRF-DAAD Masters Scholarship 2014"},{"name":"University of Kwazulu-Natal College of Health Sciences Scholarship 2014"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Ochratoxin A (OTA) is a nephrotoxic mycotoxin produced by <jats:italic>Aspergillus<\/jats:italic> and <jats:italic>Penicillium<\/jats:italic> fungi. It contaminates human and animal food products, and chronic exposure is associated with renal fibrosis in humans (Balkan endemic nephropathy). Resveratrol, a phytoalexin, possesses anti\u2010cancer and antioxidant properties. We investigated the mechanism of cellular oxidative stress induced by OTA, and the effect of resveratrol in human embryonic kidney (HEK293) cells over 24 and 48\u2009h. Cells were exposed to OTA [IC50\u2009=\u20091.5\u2009\u03bcM (24\u2009h) and 9.4\u2009\u03bcM (48\u2009h) determined using MTT assay] and 25\u2009\u03bcM resveratrol. Glutathione was quantified by luminometry and gene expression of Nrf2 and OGG1 was determined by qPCR. Protein expression of Nrf2, LonP1, SIRT3, and pSIRT1 was assessed by Western blot, DNA damage (comet assay), and intracellular reactive oxygen species (flow cytometry). At 24\u2009h, resveratrol increased mRNA expression of the DNA repair enzyme, OGG1 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), whereas OTA and OTA+resveratrol significantly decreased OGG1 expression (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). OGG1 expression increased during 48\u2010h exposure to resveratrol and OTA+resveratrol (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Comet tail lengths doubled in 48\u2010h OTA\u2010treated cells, whereas at both time periods, OTA+resveratrol yielded shorter comet tails (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). During 24\u2010 and 48\u2010h exposure, OTA, resveratrol, and OTA+resveratrol significantly decreased mRNA expression of Nrf2 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Luminometry analysis of GSH revealed an increase by OTA+resveratrol for 24 and 48\u2009h (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001, respectively). Western blot analysis showed decreased Nrf2 protein expression during 24\u2010h exposure, but increased Nrf2 expression during 48\u2009h. LonP1 protein expression increased during 24\u2010h exposure to OTA (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and OTA+resveratrol (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0011) and during 48\u2010h exposure to resveratrol (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0005). J. Cell. Biochem. 116: 2947\u20132955, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25242","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T13:18:33Z","timestamp":1434719913000},"page":"2947-2955","source":"Crossref","is-referenced-by-count":39,"title":["The Phytoalexin Resveratrol Ameliorates Ochratoxin A Toxicity in Human Embryonic Kidney (HEK293) Cells"],"prefix":"10.1002","volume":"116","author":[{"given":"Shanel","family":"Raghubeer","sequence":"first","affiliation":[{"name":"Discipline of Medical Biochemistry School of Laboratory Medicine and Medical Sciences College of Health Sciences University of KwaZulu\u2010Natal Durban 4041 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savania","family":"Nagiah","sequence":"additional","affiliation":[{"name":"Discipline of Medical Biochemistry School of Laboratory Medicine and Medical Sciences College of Health Sciences University of 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