{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T11:03:15Z","timestamp":1784718195223,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,11,24]],"date-time":"2015-11-24T00:00:00Z","timestamp":1448323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Strategic Research Program for Brain Sciences, Ministry of Education, Culture, Sports, Science, and Technology of Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>The neurohypophysial hormone oxytocin (OXT) and its receptor (OXTR) have critical roles in the regulation of pro\u2010social behaviors, including social recognition, pair bonding, parental behavior, and stress\u2010related responses. Supporting this hypothesis, a portion of patients suffering from autism spectrum disorder have mutations, such as single nucleotide polymorphisms, or epigenetic modifications in their OXTR gene. We previously reported that OXTR\u2010deficient mice exhibit pervasive social deficits, indicating the critical role of OXTR in social behaviors. In the present study, we generated <jats:italic>Oxtr cDNA<jats:sup>HA<\/jats:sup>\u2010Ires\u2010Cre<\/jats:italic> knock\u2010in mice, expressing both OXTR and Cre recombinase under the control of the endogenous <jats:italic>Oxtr<\/jats:italic> promoter. Knock\u2010in cassette of <jats:italic>Oxtr cDNA<jats:sup>HA<\/jats:sup>\u2010Ires\u2010Cre<\/jats:italic> consisted of <jats:italic>Oxtr<\/jats:italic> cDNA tagged with the hemagglutinin epitope at the 3\u2032 end (<jats:italic>Oxtr cDNA<jats:sup>HA<\/jats:sup><\/jats:italic>), internal ribosomal entry site (Ires), and <jats:italic>Cre<\/jats:italic>. Cre was expressed in the uterus, mammary gland, kidney, and brain of <jats:italic>Oxtr cDNA<jats:sup>HA<\/jats:sup>\u2010Ires\u2010Cre<\/jats:italic> knock\u2010in mice. Furthermore, the distribution of Cre in the brain was similar to that observed in <jats:italic>Oxtr\u2010Venus<\/jats:italic> fluorescent protein expressing mice (<jats:italic>Oxtr\u2010Venus<\/jats:italic>), another animal model previously generated by our group. Social behavior of <jats:italic>Oxtr cDNA<jats:sup>HA<\/jats:sup>\u2010Ires\u2010Cre<\/jats:italic> knock\u2010in mice was similar to that of wild\u2010type animals. We demonstrated that this construct is expressed in OXTR\u2010expressing neurons specifically after an infection with the recombinant adeno\u2010associated virus carrying the flip\u2010excision switch vector. Using this system, we showed the transport of the wheat\u2010germ agglutinin tracing molecule from the OXTR\u2010expressing neurons to the innervated neurons in knock\u2010in mice. This study might contribute to the monosynaptic analysis of neuronal circuits and to the optogenetic analysis of neurons expressing OXTR. J. Cell. Biochem. 117: 1099\u20131111, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25393","type":"journal-article","created":{"date-parts":[[2015,10,7]],"date-time":"2015-10-07T12:06:39Z","timestamp":1444219599000},"page":"1099-1111","source":"Crossref","is-referenced-by-count":38,"title":["Generation of <i>Oxtr cDNA<sup>HA<\/sup>\u2010Ires\u2010Cre<\/i> Mice for Gene Expression in an Oxytocin Receptor Specific Manner"],"prefix":"10.1002","volume":"117","author":[{"given":"Shizu","family":"Hidema","sequence":"first","affiliation":[{"name":"Department of Molecular Biology Graduate School of Agricultural Science Tohoku University Sendai Miyagi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomokazu","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding and Genetics Graduate School of Agricultural Science Tohoku University Sendai Miyagi 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