{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:47:42Z","timestamp":1791830862578,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T00:00:00Z","timestamp":1447718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Defense Grant","award":["PC094680"],"award-info":[{"award-number":["PC094680"]}]},{"name":"Prostate Cancer Foundation Creativity Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Despite progression in diagnosis and treatment, prostate cancer (PCa) still represents the main cause of cancer\u2010related mortality and morbidity in men. Although radiation therapy offers clinical benefit over other therapeutic modalities, the success of this therapeutic modality is commonly hampered by the resistance of advanced tumors. So far, the mechanisms governing tumor resistance to radiotherapy are not discussed in detail. Here, we demonstrate for the first time that the resistance of PCa to radiation therapy is attributed to elevated expression of Hepatoma Up\u2010Regulated Protein (HURP). In PCa cells, the induction of HURP expression suppresses \u03b3\u2010irradiation\u2010induced apoptosis. \u03b3\u2010irradiation\u2010induced apoptosis of PCa cells is associated with expression of E2F1, p53, p21 proteins together with the phosphorylation of apoptosis signal\u2010regulating kinase1 (ASK1), c\u2010jun\u2010N\u2010terminal kinase (JNK) and Ataxia\u2010telangiectasia mutated (ATM) and histone family member X (H2AX). Whereas, the induction of HURP expression is able to suppress \u03b3\u2010irradiation\u2010induced effects on E2F1, p53, p21, ATM, ASK1, JNK and ATM, and H2AX. Also, inhibition of \u03b3\u2010irradiation\u2010induced\u2010 cytochrome c release, cleavage of caspase\u20109, caspase\u20103, PARP, and reactive oxygen species (ROS) were noted in PCa cells induced for HURP expression. The observed radio\u2010resistance of PCa is thought to be the consequence of HURP\u2010mediated destabilization of p53 and ATM proteins that are essential for the modulation of \u03b3\u2010irradiation\u2010induced apoptosis. Thus, based on our findings, PCa resistance to radiation therapy results from the deregulation of ASK1\/ JNK; ATM\/ H2AX; ATM\/p53 and checkpoint kinase 2 (Chk2)\/ E2F\u20101 in response to the elevated expression of HURP. J. Cell. Biochem. 117: 1308\u20131318, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25419","type":"journal-article","created":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T01:42:44Z","timestamp":1445996564000},"page":"1308-1318","source":"Crossref","is-referenced-by-count":16,"title":["Elevated Expression of Hepatoma Up\u2010Regulated Protein Inhibits \u03b3\u2010Irradiation\u2010Induced Apoptosis of Prostate Cancer Cells"],"prefix":"10.1002","volume":"117","author":[{"given":"Mohamed","family":"Hassan","sequence":"first","affiliation":[{"name":"Cancer Institute University of Mississippi Medical Center 2500 N State St. Jackson Mississippi 39216"},{"name":"Department of Pathology University of Mississippi Medical Center 2500 N State St. Jackson Mississippi 39216"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelouahid","family":"El Khattouti","sequence":"additional","affiliation":[{"name":"Cancer Institute University of 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39216"},{"name":"Department of Pathology University of Mississippi Medical Center 2500 N State St. Jackson Mississippi 39216"},{"name":"Department of Radiation Oncology University of Mississippi Medical Center 2500 N State St. Jackson Mississippi 39216"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2001.6570"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2014.09.019"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.05.021"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104279"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026323"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(02)01672-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(02)02730-X"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/09553002.2013.775530"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.18.5678"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-013-0804-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2013.10.022"},{"key":"e_1_2_6_13_1","first-page":"5627","article-title":"Transforming 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