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Further understanding of the cellular and molecular mechanisms of V\u2010ATPase inhibition is vital for the development of anti\u2010resorptive drugs specifically targeting osteoclast V\u2010ATPases. In this study, we observed that bafilomycin A1, a naturally\u2010occurring inhibitor of V\u2010ATPases, increased the protein level of SQSTM1\/p62, a known negative regulator of osteoclast formation. Consistently, we found that bafilomycin A1 diminishes the intracellular accumulation of the acidotropic probe lysotracker in osteoclast\u2010like cells; indicative of reduced acidification. Further, bafilomycin A1 inhibits osteoclast formation with attenuation of cell fusion and multi\u2010nucleation of osteoclast\u2010like cells during osteoclast differentiation. Taken together, these data indicate that bafilomycin A1 attenuates osteoclast differentiation in part via increased levels of SQSTM1\/p62 protein, providing further mechanistic insight into the effect of V\u2010ATPase inhibition in osteoclasts. J. Cell. Biochem. 117: 1464\u20131470, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25442","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T14:20:38Z","timestamp":1447424438000},"page":"1464-1470","source":"Crossref","is-referenced-by-count":13,"title":["Bafilomycin A1 Attenuates Osteoclast Acidification and Formation, Accompanied by Increased Levels of SQSTM1\/p62 Protein"],"prefix":"10.1002","volume":"117","author":[{"given":"Sipin","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital Wenzhou Medical University Wenzhou Zhejiang 325035 China"},{"name":"School of Pathology and Laboratory Medicine The University of Western Australia Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L.","family":"Rea","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Endocrinology Harry Perkins Institute of Medical Research and UWA Centre for Medical Research The University of Western Australia Crawley WA 6009 Australia"},{"name":"Department of Endocrinology and Diabetes Sir Charles Gairdner Hospital Nedlands WA 6009 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taksum","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Surgery Centre of Orthopaedic Research The University of Western Australia Crawley WA 6009 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao Tian","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Pathology and Laboratory Medicine The University of Western Australia Crawley WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Diabetes Sir Charles Gairdner Hospital Nedlands WA 6009 Australia"},{"name":"School of Medicine and Pharmacology The 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