{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:12Z","timestamp":1786978092449,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T00:00:00Z","timestamp":1460592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"SECTyP, Universidad Nacional de Cuyo, Mendoza (Argentina)","award":["06\/J459"],"award-info":[{"award-number":["06\/J459"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that \u03b2\u2010galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N\u2010acetyl\u2010\u03b2\u2010D\u2010glucosaminidase and \u03b2\u2010glucuronidase with spermatozoa appeared to be stronger. On the other hand, \u03b1\u2010mannosidase and \u03b1\u2010fucosidase cannot be removed from the gametes. In addition, part of N\u2010acetyl\u2010\u03b2\u2010D\u2010glucosaminidase, \u03b2\u2010galactosidase, and \u03b2\u2010glucuronidase can also be released by mannose\u20106\u2010phosphate. Taking into account these data, we explored the presence of cation\u2010independent\u2010 and cation\u2010dependent\u2010mannose\u20106\u2010phosphate receptors in the spermatozoa and found that cation\u2010independent mannose\u20106\u2010phosphate receptor is highly expressed in bull spermatozoa and cation\u2010dependent\u2010mannose\u20106\u2010phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation\u2010independent\u2010mannose\u20106\u2010phosphate receptor is mostly located at the acrosomal zone, whereas cation\u2010dependent\u2010mannose\u20106\u2010phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N\u2010acetyl\u2010\u03b2\u2010D\u2010glucosaminidase and \u03b2\u2010glucuronidase isolated from epididymal fluid interacted mostly with cation\u2010independent\u2010mannose\u20106\u2010phosphate receptor, while \u03b2\u2010galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos\u20106\u2010phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464\u20132472, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.25538","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T16:16:30Z","timestamp":1457799390000},"page":"2464-2472","source":"Crossref","is-referenced-by-count":5,"title":["Glycosidases Interact Selectively With Mannose\u20106\u2010Phosphate Receptors of Bull Spermatozoa"],"prefix":"10.1002","volume":"117","author":[{"given":"Andrea C.","family":"Aguilera","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Fisiolog\u00eda Celular \u201cDr. Franciso Bertini,\u201d Instituto de Histolog\u00eda y Embriolog\u00eda\u2014CONICET\u2010FCM\u2010UNCuyo Facultad de Ciencias Exactas y Naturales\u2014Universidad Nacional de Cuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Boschin","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Fisiolog\u00eda Celular \u201cDr. Franciso Bertini,\u201d Instituto de Histolog\u00eda y Embriolog\u00eda\u2014CONICET\u2010FCM\u2010UNCuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Carvelli","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Fisiolog\u00eda Celular \u201cDr. Franciso Bertini,\u201d Instituto de Histolog\u00eda y Embriolog\u00eda\u2014CONICET\u2010FCM\u2010UNCuyo Facultad de Ciencias Exactas y Naturales\u2014Universidad Nacional de Cuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Cavicchia","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Fisiolog\u00eda Celular \u201cDr. Franciso Bertini,\u201d Instituto de Histolog\u00eda y Embriolog\u00eda\u2014CONICET\u2010FCM\u2010UNCuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Sosa","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Fisiolog\u00eda Celular \u201cDr. Franciso Bertini,\u201d Instituto de Histolog\u00eda y Embriolog\u00eda\u2014CONICET\u2010FCM\u2010UNCuyo Facultad de Ciencias Exactas y Naturales\u2014Universidad Nacional de Cuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,14]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"118","volume-title":"Lysosomes: A laboratory handbook","author":"Barrett AJ","year":"1977"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.07.013"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2605.1998.00124.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.4.1172"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/014850102753385215"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.10.016"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001273200"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22622"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(92)90082-I"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi7011806"},{"key":"e_1_2_6_12_1","first-page":"119","article-title":"Interactions between epididymal secretions and spermatozoa","volume":"53","author":"Cooper TG","year":"1998","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10314"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmn055"},{"key":"e_1_2_6_15_1","first-page":"81","volume-title":"The epididymis: From molecules to clinical practice","author":"Cuasnicu P","year":"2002"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.015"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2009.03.007"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/19396368.2012.663233"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn061"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"21687","DOI":"10.1016\/S0021-9258(18)54691-3","article-title":"The binding specificity of high and low molecular weight phosphomannosyl receptors from bovine testes. 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