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Seven peptides were constructed from the kringle 2 sequence, based on the structure and characteristics of amino acid residues, and were analyzed for their inhibitory effects on endothelial cells (ECs). Among them, TP\u20107 (derived from a \u03b2\u2010sheet motif) potently inhibited proliferation, tube formation, and migration of ECs in a dose\u2010dependent manner, whereas truncation of 3\u20139 amino acid residues from either N or C terminus of TP\u20107 abrogated its inhibitory effects on ECs. TP\u20107 also potently inhibited angiogenesis in a Matrigel plug assay in vivo. Moreover, TP\u20107 dose\u2010dependently suppressed corneal neovascularization induced by an acute chemical burn in a rat model. At the molecular level, TP\u20107 inhibited VEGF\u2010 or bFGF\u2010induced phosphorylation of FAK and ERK1\/2 and drastically disrupted VEGF\u2010 or bFGF\u2010induced formation of stress fibers and focal adhesion complexes. In addition, TP\u20107 markedly suppressed attachment and spreading of ECs on a collagen type I or fibronectin matrix. Adhesion of ECs to immobilized TP\u20107 increased dose\u2010dependently, which was disrupted strongly by pretreatment with soluble TP\u20107 and slightly by an integrin \u03b12\u03b21\u2010blocking antibody. These results suggest that TP\u20107 is a potent anti\u2010angiogenic peptide in part affecting the integrin \u03b12\u03b21\u2010dependent pathway and that it can be used for treatment of corneal neovascularization by targeting VEGF and non\u2010VEGF pathways. J. Cell. Biochem. 118: 1132\u20131143, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25732","type":"journal-article","created":{"date-parts":[[2016,9,12]],"date-time":"2016-09-12T22:54:09Z","timestamp":1473720849000},"page":"1132-1143","source":"Crossref","is-referenced-by-count":5,"title":["A Novel Peptide Derived From Tissue\u2010Type Plasminogen Activator Potently Inhibits Angiogenesis and Corneal Neovascularization"],"prefix":"10.1002","volume":"118","author":[{"given":"Hyun\u2010Kyung","family":"Kim","sequence":"first","affiliation":[{"name":"Cancer Research Institute College of Medicine The Catholic University of Korea Seoul Republic of Korea"},{"name":"Department of Medical Lifescience College of Medicine The Catholic University of Korea Seoul Republic of Korea"},{"name":"Cancer Evolution Research Center College of Medicine The Catholic University of Korea Seoul Republic of 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