{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T21:43:19Z","timestamp":1778622199689,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T00:00:00Z","timestamp":1482192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>ClC\u20103 chloride channel has been proved to have a relationship with the expression of osteogenic markers during osteogenesis, persistent static compression can upregulate the expression of ClC\u20103 and regulate osteodifferentiation in osteoblasts. However, there was no study about the relationship between the expression of ClC\u20103 and osteodifferentiation after dynamic compression. In this study, we applied dynamic compression on MC3T3\u2010E1 cells to detect the expression of ClC\u20103, runt\u2010related transcription factor 2 (<jats:italic>Runx2<\/jats:italic>), bone morphogenic protein\u20102 (<jats:italic>BMP\u20102<\/jats:italic>), osteopontin (<jats:italic>OPN<\/jats:italic>), nuclear\u2010associated antigen Ki67 (<jats:italic>Ki67<\/jats:italic>), and proliferating cell nuclear antigen (<jats:italic>PCNA<\/jats:italic>) in biopress system, then we investigated the expression of these genes after dynamic compression with Chlorotoxin (specific ClC\u20103 chloride channel inhibitor) added. Under transmission electron microscopy, there were more cell surface protrusions, rough surfaced endoplasmic reticulum, mitochondria, Golgi apparatus, abundant glycogen, and lysosomes scattered in the cytoplasm in MC3T3\u2010E1 cells after dynamic compression. The nucleolus was more obvious. We found that ClC\u20103 was significantly up\u2010regulated after dynamic compression. The compressive force also up\u2010regulated Runx2, BMP\u20102, and OPN after dynamic compression for 2, 4 and 8\u2009h. The proliferation gene Ki67 and PCNA did not show significantly change after dynamic compression for 8\u2009h. Chlorotoxin did not change the expression of ClC\u20103 but reduced the expression of Runx2, BMP\u20102, and OPN after dynamic compression compared with the group without Cltx added. The data from the current study suggested that ClC\u20103 may promotes osteogenic differentiation in MC3T3\u2010E1 cell after dynamic compression. J. Cell. Biochem. 118: 1606\u20131613, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.25823","type":"journal-article","created":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T11:06:46Z","timestamp":1481022406000},"page":"1606-1613","source":"Crossref","is-referenced-by-count":16,"title":["ClC\u20103 Promotes Osteogenic Differentiation in MC3T3\u2010E1 Cell After Dynamic Compression"],"prefix":"10.1002","volume":"118","author":[{"given":"Dawei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Stomatology Third Hospital of Hebei Medical University Shijiazhuang 050051 Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Third Hospital of Hebei Medical University Shijiazhuang 050051 Hebei 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