{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:36:24Z","timestamp":1769063784530,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:00:00Z","timestamp":1494892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Nicotinic acid adenine dinucleotide phosphate (NAADP) is the newest discovered intracellular second messengers, which is able to release Ca<jats:sup>2+<\/jats:sup> stored within endolysosomal (EL) vesicles. NAADP\u2010induced Ca<jats:sup>2+<\/jats:sup> signals mediate a growing number of cellular functions, ranging from proliferation to muscle contraction and differentiation. Recently, NAADP has recently been shown to regulate angiogenesis by promoting endothelial cell growth. It is, however, still unknown whether NAADP stimulates proliferation also in endothelial progenitor cells, which are mobilized in circulation after an ischemic insult to induce tissue revascularization. Herein, we described a novel approach to prepare NAADP\u2010containing liposomes, which are highly cell membrane permeable and are therefore amenable for stimulating cell activity. Accordingly, NAADP\u2010containing liposomes evoked an increase in intracellular Ca<jats:sup>2+<\/jats:sup> concentration, which was inhibited by NED\u201019, a selective inhibitor of NAADP\u2010induced Ca<jats:sup>2+<\/jats:sup> release. Furthermore, NAADP\u2010containing liposomes promoted EPC proliferation, a process which was inhibited by NED\u201019 and BAPTA, a membrane permeable intracellular Ca<jats:sup>2+<\/jats:sup> buffer. Therefore, NAADP\u2010containing liposomes stand out as a promising tool to promote revascularization of hypoxic\/ischemic tissues by favoring EPC proliferation. J. Cell. Biochem. 118: 3722\u20133729, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26019","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T16:38:37Z","timestamp":1491410317000},"page":"3722-3729","source":"Crossref","is-referenced-by-count":28,"title":["Liposomes as a Putative Tool to Investigate NAADP Signaling in Vasculogenesis"],"prefix":"10.1002","volume":"118","author":[{"given":"Francesca","family":"Di Nezza","sequence":"first","affiliation":[{"name":"Department of Bioscience and Territory (DIBT) University of Molise Contrada Lappone Pesche Isernia 86090 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estella","family":"Zuccolo","sequence":"additional","affiliation":[{"name":"Laboratory of General Physiology Department of Biology and Biotechnology \u201cL. Spallanzani\u201d University of Pavia Pavia 27100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Poletto","sequence":"additional","affiliation":[{"name":"Biotechnology Research Laboratory Center for the Study of Myelofibrosis Fondazione IRCCS Policlinico San Matteo Pavia 27100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittorio","family":"Rosti","sequence":"additional","affiliation":[{"name":"Biotechnology Research Laboratory Center for the Study of Myelofibrosis Fondazione IRCCS Policlinico San Matteo Pavia 27100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"De Luca","sequence":"additional","affiliation":[{"name":"Department of Mental and Physical Health and Preventive Medicine Section of Human Anatomy Universit\u00e0 degli Studi della Campania \u201cL. Vanvitelli\u201d Naples 80138 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0010-0098","authenticated-orcid":false,"given":"Francesco","family":"Moccia","sequence":"additional","affiliation":[{"name":"Laboratory of General Physiology Department of Biology and Biotechnology \u201cL. Spallanzani\u201d University of Pavia Pavia 27100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germano","family":"Guerra","sequence":"additional","affiliation":[{"name":"Department of Medicine and Health Sciences \u201cVincenzo Tiberio\u201d Centre of Nanomedicine University of Molise Campobasso 86100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Ambrosone","sequence":"additional","affiliation":[{"name":"Department of Medicine and Health Sciences \u201cVincenzo Tiberio\u201d Centre of Nanomedicine University of Molise Campobasso 86100 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7757(03)00271-1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/la990257e"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24423"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408746200"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030472"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2016.12.160"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1035-x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/18032"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.01.024"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.06.017"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1088\/0022-3727\/42\/2\/025401"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2015.09.002"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406029111"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.206698"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2014.09.010"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot076927"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2010.11.011"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/17435889.1.3.297"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/la00002a020"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.222099799"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.6030\/1939-067X-4.1.16"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/la048110x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.09.007"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2017.01.025"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25126"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0178fje"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/157016111201140327162858"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.02.011"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450116666141219121723"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110949"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2007.08.006"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2015.03.006"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2015.01.001"},{"key":"e_1_2_5_35_1","first-page":"123","article-title":"Fate and behavior of liposomes in vivo: A review of controlling factors","volume":"3","author":"Senior JH","year":"1987","journal-title":"Crit Rev Ther Drug Carrier Syst"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2013-304498"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2016.0050"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aab0863"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2015.0240"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2016.09.005"},{"key":"e_1_2_5_41_1","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.vph.2015.11.082","article-title":"Acidic Ca2+ stores interact with the endoplasmic reticulum to shape intracellular Ca2+ signals in human endothelial progenitor cells","volume":"75","author":"Zuccolo E","year":"2015","journal-title":"Vascul Pharmacol"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.26019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.26019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.26019","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.26019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T12:26:05Z","timestamp":1693398365000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.26019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,16]]},"references-count":40,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/jcb.26019"],"URL":"https:\/\/doi.org\/10.1002\/jcb.26019","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,16]]}}}