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However, the underlying anticancer mechanisms have not previously been identified. The proliferation of A549 was assessed by MTT assay. The reactive oxygen species (ROS) level was assessed in A549 with a H<jats:sub>2<\/jats:sub>DCFDA probe. Mitochondrial membrane potential was measured using the JC\u20101 staining. Apoptosis were measured by annexin\u2010V\/PI assay and autophagy by acridine orange staining and GFP\u2010LC3 fluorescence assay. The expression of autophagic and apoptotic proteins was determined by Western blot. The compound HC6h increased accumulation of vesicles, acridine orange\u2010stained cells and LC3\u2010II in A549 cells. Inhibition of compound HC6h\u2010induced autophagy by bafilomycin A1 increased apoptosis. Compound HC6h enhanced activation of caspase\u20103, caspase\u20109 and PARP cleavage in A549 cells. Compound HC6h leads to the rapid generation of intracellular ROS. Moreover, compound HC6h induced phosphorylation of JNK and was conferred by the increased ROS levels. Furthermore, down\u2010regulation of JNK attenuated autophagic and apoptotic effect in response to HC6h. The induction of ROS upon HC6h treatment leads to the activation of JNK that mediates autophagy and apoptosis in human A549 cancer cells. J. Cell. Biochem. 118: 4394\u20134403, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26093","type":"journal-article","created":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T11:53:50Z","timestamp":1493207630000},"page":"4394-4403","source":"Crossref","is-referenced-by-count":1,"title":["An Isoquinolin\u20101(2<i>H<\/i>)\u2010Imine Derivative Induces Cell Death via Generation of Reactive Oxygen Species and Activation of JNK in Human A549 Cancer Cells"],"prefix":"10.1002","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-1952","authenticated-orcid":false,"given":"Jing","family":"Liu","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Genetics of Aging and Tumor Faculty of Medicine Kunming University of Science and Technology Kunming 650500 P.R. 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