{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T14:00:54Z","timestamp":1776952854744,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M592825"],"award-info":[{"award-number":["2016M592825"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004572","name":"National Health and Family Planning Commission of the People's Republic of China","doi-asserted-by":"publisher","award":["2016D044"],"award-info":[{"award-number":["2016D044"]}],"id":[{"id":"10.13039\/501100004572","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Picroside II, one of the major components isolated from the seed of natural plant picrorhiza, is widely used in traditional Chinese medicine. The present study was performed to define effects of picroside II on nuclear factor\u2010kappaB ligand (RANKL)\u2010stimulated osteoclast differentiation in vitro and on lipopolysaccharide (LPS)\u2010induced bone loss in vivo. The bone marrow cells (BMMs) were harvested and induced with RANKL followed by treatment with picroside II at several doses, and the differentiation of osteoclasts from these cells was evaluated by tartrate\u2010resistant acid phosphatase (TRAP) staining and resorption pit formation assay. The effects of picroside II on osteoclastogenesis were studied by examining RANKL\u2010induced osteoclast F\u2010actin ring formation and osteoclast bone resorption. Moreover, we explored the mechanisms of these downregulation effects by performed Western blotting and quantitative RT\u2010PCR examination. Results demonstrated picroside II strongly inhibited RANKL\u2010induced osteoclast formation when added during the early stage of BMMs cultures, suggesting that it acts on osteoclast precursors to inhibit RANKL\/RANK signaling. Moreover, picroside II markedly decreased the phosphorylation of p38, ERK, JNK, p65, and I\u2010\u03baB degradation, and significantly suppressed c\u2010Fos and nuclear factor of activated T\u2010cells cytoplasmic 1 (NFATc1), both the key transcription factors during osteoclastogenesis. Furthermore, in vivo studies verified the bone protection effects of picroside II. These results collectively suggested that picroside II acted as an anti\u2010resorption agent by blocking osteoclast activation. J. Cell. Biochem. 118: 4479\u20134486, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26105","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T17:32:37Z","timestamp":1493746357000},"page":"4479-4486","source":"Crossref","is-referenced-by-count":16,"title":["Picroside II Inhibits RANKL\u2010Mediated Osteoclastogenesis by Attenuating the NF\u2010\u03baB and MAPKs Signaling Pathway In Vitro and Prevents Bone Loss in Lipopolysaccharide Treatment Mice"],"prefix":"10.1002","volume":"118","author":[{"given":"Xiaobin","family":"Yang","sequence":"first","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Gao","sequence":"additional","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biao","family":"Wang","sequence":"additional","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Wang","sequence":"additional","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3655-3171","authenticated-orcid":false,"given":"Lingbo","family":"Kong","sequence":"additional","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dingjun","family":"Hao","sequence":"additional","affiliation":[{"name":"Hong\u2010Hui Hospital Xi'an Jiaotong University College of Medicine Xi'an 710054 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119679"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.12.1741"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.10.103"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.12.012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7939685"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-9081-6-43"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208284200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1197-1285"},{"issue":"6","key":"e_1_2_8_11_1","first-page":"3507","article-title":"Picroside II protects myocardium from ischemia\/reperfusion\u2010induced injury through inhibition of the inflammatory response","volume":"12","author":"Jian L","year":"2015","journal-title":"Exp Ther Med"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505815200"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.6.1271"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061013"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001229200"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313973200"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2007.05.016"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-009-9316-6"},{"key":"e_1_2_8_19_1","first-page":"28","article-title":"Immunopharmacological studies on Picrorhiza kurroa Royle\u2010ex\u2010Benth. 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