{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:49:22Z","timestamp":1790210962014,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T00:00:00Z","timestamp":1499126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>This study aims to explore the effects of shRNA\u2010mediated silencing on Pyruvate kinase type M2 (PKM2) gene during aerobic glycolysis in colorectal cancer (CRC) cells. CRC tissues and adjacent normal tissues were obtained from 136 patients diagnosed with qRT\u2010PCR, Western blotting, and immunohistochemistry (IHC) were performed to detect mRNA and protein expressions of PKM2. CRC cells were divided into a blank, vector, and PKM2\u2010shRNA groups. Hexokinase (HK) and PKM2 activity were both determined by glucose\u20106\u2010phosphate dehydrogenase (G\u20106\u2010PD) coupled colorimetric assay and enzyme coupling rate method. The extracellular lactate concentration was measured by ultraviolet spectrophotometer and caspase activity was measured using spectrophotometry. The proliferation, cell cycle, apoptosis, invasion, and migration of CRC cells were detected by cell counting kit\u20108 (CCK\u20108) assay, flow cytometry, transwell assay, and scratch test. Three groups of nude mice were injected with 0.2\u2009mL single\u2010cell suspension from the blank, vector, and PKM2\u2010shRNA groups, respectively. PKM2 protein content in CRC tissues was higher than that in adjacent normal tissues. Results showed that the PKM2\u2010shRNA group exhibited significantly lower mRNA and protein expressions of PKM2, decreased PKM2 activity, reduced lactate metabolism level, increased cell apoptosis rate, elevated caspase\u20103 and caspase\u20109 activity, weakened proliferation, and a reduction in cell invasion and migration ability compared to the vector and blank groups. The optical density (OD) value was lower in the PKM2\u2010shRNA group than in the blank and vector groups. These findings indicate that shRNA\u2010mediated silencing of PKM2 gene promotes apoptosis and inhibits aerobic glycolysis, proliferation, migration, and invasion in CRC cells. J. Cell. Biochem. 118: 4792\u20134803, 2017. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26148","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T14:25:27Z","timestamp":1495549527000},"page":"4792-4803","source":"Crossref","is-referenced-by-count":24,"title":["<i>Retracted<\/i>: Effects of shRNA\u2010Mediated Silencing of PKM2 Gene on Aerobic Glycolysis, Cell Migration, Cell Invasion, and Apoptosis in Colorectal Cancer Cells"],"prefix":"10.1002","volume":"118","author":[{"given":"Xiao\u2010Ling","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Pathology Tianjin Huanhu Hospital Tianjin 300350 P. R. China"},{"name":"Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases Tianjin 300350 P. R. 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