{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:40:49Z","timestamp":1790228449951,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T00:00:00Z","timestamp":1499126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      An interaction between\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      and macrophages constitutes an essential step in tuberculosis development, as macrophages exert both positive and negative effects on\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      \u2010triggered organ lesions. In this study, we focused on the regulation of the expression of matrix metalloproteinases (MMPs), which is responsible for lung matrix degradation and bacteria dissection, in macrophages following\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      infection. Female BALB\/c mice were intravenously injected with the\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      strain H37Rv at 0\u2009h zeitgeber time (ZT0) or 12\u2009h zeitgeber time (ZT12). The expression and activity of MMP\u20101, \u20102, \u20103, and \u20109 in lungs and spleens were then evaluated. In vitro, peritoneal macrophages were harvested at ZT0 or at ZT12 and infected with 10 MOI\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      . The expression of MMPs, microRNA\u2010223 and BMAL1 was analyzed by qRT\u2010PCR and\/or Western blot. The binding of BMAL1 3\u2032\u2010UTR by miR\u2010223 was confirmed by luciferase activity assay. Additionally, wild\u2010type BMAL1 or NLS\n                      <jats:sup>mut<\/jats:sup>\n                      BMAL1 plasmids were transfected to evaluate the effect of BMAL1 on MMPs. The results showed a differential expression of MMPs in mice tissues infected at different times.\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      infection caused enhanced MMP\u20101, \u20109, and miR\u2010223 expression, with inhibited BMAL1 expression. MiR\u2010223 modulated BMAL1 expression via the direct binding of BMAL1 3\u2032\u2010UTR. Furthermore, wild\u2010type BMAL1 other than NLS\n                      <jats:sup>mut<\/jats:sup>\n                      BMAL1 attenuated MMPs expression in\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      \u2010infected macrophages. Overall, this study demonstrated a potential involvement of circadian rhythm in MMP activation by\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                      in macrophages. J. Cell. Biochem. 118: 4804\u20134812, 2017. \u00a9 2017 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.26150","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T10:24:18Z","timestamp":1495535058000},"page":"4804-4812","source":"Crossref","is-referenced-by-count":28,"title":["Activation of MMPs in Macrophages by\n                    <i>Mycobacterium tuberculosis<\/i>\n                    via the miR\u2010223\u2010BMAL1 Signaling Pathway"],"prefix":"10.1002","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7307-5746","authenticated-orcid":false,"given":"Jun","family":"Lou","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Zhumadian Central Hospital Zhumadian 463000 P.R. 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