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Human mesenchymal stem cells were transduced with an adenoviral vector containing the cDNA for bone morphogenetic protein 2 (AdBMP2) to induce bone formation. To make this method safer, a system to quickly kill these virally transduced cells was designed and evaluated. Cells were encapsulated inside poly(ethylene glycol) diacrylate (PEG\u2010Da) hydrogels that are able to shield the cells from immunological destruction. The system involves an inducible caspase\u20109 (iCasp9) activated using a specific chemical inducer of dimerization (CID). Delivering AdBMP2\u2010transduced human mesenchymal stem cells encapsulated in PEG\u2010Da hydrogel promoted ectopic ossification in vivo, and the iCasp9 system allowed direct control of the timing of apoptosis of the injected cells. The iCasp9\u2010CID system enhances the safety of delivering AdBMP2\u2010transduced cells, making it a more compelling therapeutic for bone repair and spine fusion. J. Cell. 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