{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:22:37Z","timestamp":1792002157005,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T00:00:00Z","timestamp":1504742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP15H06451"],"award-info":[{"award-number":["JP15H06451"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17K17352"],"award-info":[{"award-number":["JP17K17352"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Accumulation of advanced glycation end\u2010products (AGEs) in periodontal tissues of patients with diabetes mellitus aggravates periodontitis, but the mechanisms are unknown. Calprotectin, a heterocomplex of S100A8 and S100A9 proteins, is a constitutive cytoplasmic component of healthy gingival epithelial cells. This study aimed at investigating the effects of AGE and <jats:italic>Porphyromonas gingivalis<\/jats:italic> lipopolysaccharide (PgLPS) on calprotectin expression in the human gingival epithelial cell line OBA\u20109. AGE and PgLPS increased the expression of S100A8 and S100A9 mRNAs, and AGE+PgLPS co\u2010stimulation amplified their expression in OBA\u20109 cells. A higher concentration of calprotectin in cell lysates was also induced by stimulation with AGE and\/or PgLPS. S100A8 was mainly translocated from the nucleus to the cytoplasm by AGE stimulation, while cytoplasmic localization of S100A9 was not altered following stimulation with AGE and\/or PgLPS. Calprotectin was found in the cytoplasm of BSA\u2010treated cells, but cytoplasmic and nuclear localization was observed following stimulation with AGE and\/or PgLPS. AGE\u2010induced S100A8, and S100A9 mRNA expression was partially suppressed by RAGE\u2010specific siRNA. In contrast, PgLPS\u2010induced S100A8 and S100A9 mRNA expression was strongly suppressed by TLR2\u2010specific siRNA. Furthermore, the inhibition of p38, JNK MAPK, and NF\u2010\u03baB attenuated AGE\u2010 and PgLPS\u2010induced S100A8 and S100A9 mRNA expression. Taken together, these results demonstrate that AGE acts in synergy with PgLPS to stimulate RAGE and TLR2 expression and activate p38, JNK MAPK, and NF\u2010\u03baB signaling pathways, resulting in increased activation of calprotectin (S100A8\/S100A9) in human gingival epithelial cells. Our results suggest that calprotectin may be involved in the pathogenesis of diabetic periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26319","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T16:07:58Z","timestamp":1501776478000},"page":"1591-1603","source":"Crossref","is-referenced-by-count":31,"title":["Advanced glycation end\u2010products and <i>Porphyromonas gingivalis<\/i> lipopolysaccharide increase calprotectin expression in human gingival epithelial cells"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3175-7850","authenticated-orcid":false,"given":"Yuka","family":"Hiroshima","sequence":"first","affiliation":[{"name":"Institute for Genome Research Tokushima University Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eijiro","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Endodontology, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaya","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Oral Healthcare Education, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Abe","sequence":"additional","affiliation":[{"name":"SHIBASAKI, Inc. Chichibu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Naruishi","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Endodontology, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takenori","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Institute for Genome Research Tokushima University Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Institute for Genome Research Tokushima University Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010ichi","family":"Kido","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Endodontology, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn L.","family":"Geczy","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of New South Wales Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00159.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/157339911797579205"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb01178.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1817"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.77.1.15"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.06.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.12.016"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-13-1505"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152309789838975"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1998.tb02340.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.261004.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.3.361"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","unstructured":"Kido J Kido R Suryono Kataoka M Fagerhol MK Nagata T. 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