{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T23:08:43Z","timestamp":1767136123385,"version":"build-2238731810"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T00:00:00Z","timestamp":1505692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The study elucidated the effects associated with silencing growth factor\u2010\u03b2 R1 (\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      ) and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      genes on the proliferation and apoptosis of penile urethral epithelial cells (UECs) in hypospadiac male rats. Seventy\u2010five male rats were distributed into the normal, model,\n                      <jats:italic>TGF\u2010\u03b2 R1\/2<\/jats:italic>\n                      \u2010siRNA,\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \u2010siRNA and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      \u2010siRNA groups. The UECs of the rats included in the study were cultured in vitro and subsequently divided into the control, blank,\n                      <jats:italic>TGF\u2010\u03b2 R1\/2<\/jats:italic>\n                      \u2010siRNA,\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \u2010siRNA and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      \u2010siRNA groups. The mRNA and protein expressions of\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \/\n                      <jats:italic>R<\/jats:italic>\n                      2 were measured by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) and western blotting. Cell proliferation and apoptosis were evaluated by cell counting kit\u20108 (CCK\u20108) assay as well as by flow cytometry. Compared with the normal group, the apoptotic rate of the UECs in the model,\n                      <jats:italic>TGF\u2010\u03b2 R1\/2<\/jats:italic>\n                      \u2010siRNA,\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \u2010siRNA and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      \u2010siRNA groups displayed remarkable increases; compared with the model group, the apoptotic rate of the UECs in the\n                      <jats:italic>TGF\u2010\u03b2 R1\/2<\/jats:italic>\n                      \u2010siRNA,\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \u2010siRNA and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      \u2010siRNA groups displayed significant decreases, similar observations were made regarding mRNA and protein expressions of\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      and\n                      <jats:italic>TGF\u2010\u03b2 R<\/jats:italic>\n                      2. Compared with the\n                      <jats:italic>TGF\u2010\u03b2 R1\/2<\/jats:italic>\n                      \u2010siRNA group, the apoptotic rates of the UECs in the\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \u2010siRNA and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      \u2010siRNA groups were up regulated, while cell proliferation in the\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      \u2010siRNA and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      \u2010siRNA groups decreased accompanied by an increased rate of apoptosis. This study ultimately demonstrated that the silencing of\n                      <jats:italic>TGF\u2010\u03b2 R1<\/jats:italic>\n                      and\n                      <jats:italic>TGF\u2010\u03b2 R2<\/jats:italic>\n                      genes could promote cell proliferation and inhibit apoptosis of penile UECs in hypospadiac male rats.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.26324","type":"journal-article","created":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T07:52:42Z","timestamp":1501833162000},"page":"1646-1658","source":"Crossref","is-referenced-by-count":0,"title":["<i>Retracted<\/i>\n                    : Synergistic effect of siRNA\u2010mediated silencing of\n                    <i>TGF\u2010\u03b2 R1<\/i>\n                    and\n                    <i>TGF\u2010\u03b2 R2<\/i>\n                    genes on the proliferation and apoptosis of penile urethral epithelial cells in hypospadiac male rats"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4874-0748","authenticated-orcid":false,"given":"Chao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Urology Jining No.1 People's Hospital Jining P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Yan","family":"Qiao","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Jining Medical University Jining P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2007.00243.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.721"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.02.043"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpurol.2015.12.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.2011.03340.x"},{"key":"e_1_2_7_7_1","first-page":"497","article-title":"Prevalence and risk factors of hypospadias in a private hospital in northeast iran","volume":"21","author":"Mohammadzadeh A","year":"2011","journal-title":"Iran J Pediatr"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2006.05.020"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e3182253430"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798316003624"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-247288"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgr246"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2014.03.003"},{"key":"e_1_2_7_14_1","first-page":"425","article-title":"Single vs. double layer suturing method repair of the urethral plate in the rabbit model of hypospadias","volume":"69","author":"Shirazi M","year":"2016","journal-title":"Cent European J Urol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4564"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168363"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1100\/tsw.2011.76"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.07.023"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4111\/kju.2010.51.8.561"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10934529.2012.680387"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9936"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-14-0054"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12551"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-51"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.319"}],"updated-by":[{"DOI":"10.1002\/jcb.30034","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"record-id":"30269"},{"DOI":"10.1002\/jcb.30034","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000}}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.26324","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.26324","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.26324","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.26324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T14:31:55Z","timestamp":1753799515000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.26324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,18]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/jcb.26324"],"URL":"https:\/\/doi.org\/10.1002\/jcb.26324","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,18]]}}}