{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:16:21Z","timestamp":1791000981629,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T00:00:00Z","timestamp":1504742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372852"],"award-info":[{"award-number":["81372852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572961"],"award-info":[{"award-number":["81572961"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Cancer cachexia is a devastating, multifactorial, and irreversible syndrome characterized by skeletal muscle reduction with or without fat loss. Although much attention has been focused on muscle wasting, fat loss may occur earlier and accelerate muscle wasting in cachexia. The cause of 20% of cancer related death makes it urgent to discover molecular mechanisms behind cancer cachexia. Here we applied weighted gene co\u2010expression network analysis (WGCNA) to identify cachexia related gene modules using differentially expressed 3289 genes and 59 long non\u2010coding RNAs based on microarray data of cachectic and non\u2010cachectic subcutaneous adipose tissue. Subsequently, 16 independent modules were acquired and GSAASeqSP Toolset confirmed that black module was significantly associated with fat loss in cancer cachexia. Top 50 hub\u2010genes in black module contained only one lncRNA, VLDLR antisense RNA 1 (VLDLR\u2010AS1). We then explored the function of black module from the view of VLDLR\u2010AS1\u2010connected genes in the network. GO enrichment and KEGG pathways analysis revealed LDLR\u2010AS1\u2010connected genes were involved in Wnt signaling pathway, small GTPase mediated signal transduction, epithelial\u2010mesenchymal transition and so on. Through construction of competing endogenous RNAs (ceRNAs) regulation network, we showed that VLDLR\u2010AS1 may function with hsa\u2010miR\u2010600 to regulate gene GOLGA3, DUSP14, and UCHL1, or interact with hsa\u2010miR\u20101224\u20103p to modulate the expression of gene GOLGA3, ZNF219, RNF141, and CALU. After literature validation, we predicted that VLDLR\u2010AS1 most likely interacted with miR\u2010600 to regulate UCH\u2010L1 through Wnt\/\u03b2\u2010catenin signaling pathway. However, further experiments are still required to validate mechanisms of VLDLR\u2010AS1 in fat reduction of cancer cachexia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26328","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T06:06:40Z","timestamp":1502086000000},"page":"1679-1688","source":"Crossref","is-referenced-by-count":18,"title":["Identification and functional analysis of a potential key lncRNA involved in fat loss of cancer cachexia"],"prefix":"10.1002","volume":"119","author":[{"given":"Huiquan","family":"Liu","sequence":"first","affiliation":[{"name":"Cancer Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhou","sequence":"additional","affiliation":[{"name":"Cancer Center, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan 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