{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:17:38Z","timestamp":1789013858567,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Vermont Cancer Center on behalf of the Fearons Cancer Research Fund"},{"name":"The Lake Champlain Cancer Research Organization"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:label\/><jats:p>Acute myelogenous leukemia (AML) is an aggressive hematologic cancer characterized by infiltration of proliferative, clonal, abnormally differentiated cells of myeloid lineage in the bone marrow and blood. Malignant cells in AML often exhibit chromosomal and other genetic or epigenetic abnormalities that are useful in prognostic risk assessment. In this study, the relative expression and novel single\u2010stranded DNA (ssDNA) binding function of purine\u2010rich element binding proteins A and B (Pur\u03b1 and Pur\u03b2) were systematically evaluated in established leukemia cell lines and in lineage committed myeloid cells isolated from patients diagnosed with a hematologic malignancy. Western blotting revealed that Pur\u03b1 and Pur\u03b2 are markedly elevated in CD33<jats:sup>+<\/jats:sup>\/CD66b<jats:sup>+<\/jats:sup> cells from AML patients compared to healthy subjects and to patients with other types of myeloid cell disorders. Results of in silico database analysis of <jats:italic>PURA<\/jats:italic> and <jats:italic>PURB<\/jats:italic> mRNA expression during hematopoiesis in conjunction with the quantitative immunoassay of the ssDNA\u2010binding activities of Pur\u03b1 and Pur\u03b2 in transformed leukocyte cell lines pointed to Pur\u03b2 as the more distinguishing biomarker of myeloid cell differentiation status. Pur\u03b2 ssDNA\u2010binding activity was significantly increased in myeloid cells from AML patients but not from individuals with other myeloid\u2010related diseases. The highest levels of Pur\u03b2 activity were detected in myeloid cells from primary AML patients and from AML patients displaying other risk factors forecasting a poor prognosis. Collectively, these findings suggest that the enhanced ssDNA\u2010binding activity of Pur\u03b2 in transformed myeloid cells may serve as a unique and measurable phenotypic trait for improving prognostic risk stratification in AML.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26369","type":"journal-article","created":{"date-parts":[[2017,8,23]],"date-time":"2017-08-23T09:06:44Z","timestamp":1503479204000},"page":"2073-2083","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of purine\u2010rich element binding protein B as a novel biomarker in acute myelogenous leukemia prognostication"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5863-4423","authenticated-orcid":false,"suffix":"Jr","given":"Robert J.","family":"Kelm","sequence":"first","affiliation":[{"name":"Division of Cardiovascular Medicine  Department of Medicine University of Vermont Robert Larner, M. D. 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