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Male Wistar rats were randomly selected to establish hydronephrosis rat model, after which all rats were classified into normal, model, negative control (NC), siRNA\u2010SOCS\u20101, siRNA\u2010SOCS\u20103, and siRNA\u2010SOCS\u20101\u2009+\u2009siRNA\u2010SOCS\u20103 groups. The levels of urine protein, serum creatinine (Scr), and blood urea nitrogen (BUN) were detected. ELISA was performed to determine levels of cystatin (CysC), \u03b22\u2010microglobulin (\u03b22\u2010MG), interleukin (IL)\u20106, and tumor necrosis factor (TNF)\u2010\u03b1. RT\u2010qPCR and Western blotting were used for mRNA and protein expressions of SOCS\u20101, SOCS\u20103, \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), and transforming Growth Factor (TGF)\u2010\u03b21. Compared with the normal group, the levels of Scr, BUN, urine protein, NAG, CysC, \u03b22\u2010MG, IL\u20106, and TNF\u2010\u03b1 were increased in other groups, as well as elevated mRNA and protein expressions of SOCS\u20101, SOCS\u20103, \u03b1\u2010SMA, and TGF\u2010\u03b21. The siRNA\u2010SOCS\u20101, siRNA\u2010SOCS\u20103, and siRNA\u2010SOCS\u20101\u2009+\u2009siRNA\u2010SOCS\u20103 groups were found with decreased levels of Scr, BUN, urine protein, NAG, CysC, \u03b22\u2010MG, IL\u20106, and TNF\u2010\u03b1, as well as mRNA and protein expressions of SOCS\u20101, SOCS\u20103, \u03b1\u2010SMA, and TGF\u2010\u03b21, including positive rates of SOCS\u20101 and SOCS\u20103 proteins in comparison with the model and NC groups. In comparison with the siRNA\u2010SOCS\u20101 and siRNA\u2010SOCS\u20103 groups, the siRNA\u2010SOCS\u20101\u2009+\u2009siRNA\u2010SOCS\u20103 group exhibited decreased levels of Scr, BUN, urine protein, NAG, CysC, \u03b22\u2010MG, IL\u20106, and TNF\u2010\u03b1. Our study demonstrated that silencing of SOCS\u20101\/3 may suppress RIF by alleviating the renal tubular damage in rat models affected by hydronephrosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26382","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T08:14:49Z","timestamp":1504167289000},"page":"2200-2211","source":"Crossref","is-referenced-by-count":10,"title":["Silencing of SOCS\u20101 and SOCS\u20103 suppresses renal interstitial fibrosis by alleviating renal tubular damage in a rat model of hydronephrosis"],"prefix":"10.1002","volume":"119","author":[{"given":"Gui\u2010Hong","family":"Zheng","sequence":"first","affiliation":[{"name":"Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province School of Life Science Jiangsu Normal University Xuzhou P.R. 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