{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T01:20:29Z","timestamp":1772932829078,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Reumatikerfonden, Sweden","award":["R-567071"],"award-info":[{"award-number":["R-567071"]}]},{"name":"Ume\u00e5 University, Faculty of Medicine, Sweden"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The cell\u2010based therapies could be potential methods to treat damaged cartilage tissues. Instead of native hyaline cartilage, the current therapies generate mainly weaker fibrocartilage\u2010type of repair tissue. A correct microenvironment influences the cellular phenotype, and together with external factors it can be used, for example, to aid the differentiation of mesenchymal stem cells to defined types of differentiated adult cells. In this study, we investigated the effect of long\u2010term exposure to 5% low oxygen atmosphere on human chondrosarcoma HCS\u20102\/8 cells. This atmosphere is close to normal oxygen tension of cartilage tissue. The proteome was analyzed with label\u2010free mass spectrometric method and further bioinformatic analysis. The qRT\u2010PCR method was used to gene expression analysis, and ELISA and dimethylmethylene blue assays for type II collagen and sulfated glycosaminoglycan measurements. The 5% oxygen atmosphere did not influence cell proliferation, but enhanced slightly ACAN and COL2A1 gene expression. Proteomic screening revealed a number of low oxygen\u2010induced protein level responses. Increased ones included NDUFA4L2, P4HA1, NDRG1, MIF, LDHA, PYGL, while TXNRD1, BAG2, TXN2, AQSTM1, TNFRSF1B, and EPHX1 decreased during the long\u2010term low oxygen atmosphere. Also a number of proteins previously not related to low oxygen tension changed during the treatment. Of those S100P, RPSS26, NDUFB11, CDV3, and TUBB8 had elevated levels, while ALCAM, HLA\u2010B, EIF1, and ACOT9 were lower in the samples cultured at low oxygen tension. In conclusion, low oxygen condition causes changes in the cellular amounts of several proteins.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26394","type":"journal-article","created":{"date-parts":[[2017,9,2]],"date-time":"2017-09-02T07:12:42Z","timestamp":1504336362000},"page":"2320-2332","source":"Crossref","is-referenced-by-count":21,"title":["Effects of long\u2010term low oxygen tension in human chondrosarcoma cells"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1732-4269","authenticated-orcid":false,"given":"Juha","family":"Piltti","sequence":"first","affiliation":[{"name":"Department of Integrative Medical Biology Ume\u00e5 University Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9833-4628","authenticated-orcid":false,"given":"Joakim","family":"Bygdell","sequence":"additional","affiliation":[{"name":"Department of Chemistry  Ume\u00e5 University Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1710-7715","authenticated-orcid":false,"given":"Chengjuan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology Ume\u00e5 University Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6181-9904","authenticated-orcid":false,"given":"Mikko J.","family":"Lammi","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology Ume\u00e5 University Ume\u00e5 Sweden"},{"name":"School of Public Health Health Science Center Xi'an Jiaotong University  Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission Xi'an Shaanxi P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20675"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.111211"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3210095"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24851"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2000.0396"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.04.040"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22878"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2008.04.007"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/267531a0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.1.17"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4272"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr700658q"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414271111"},{"key":"e_1_2_8_15_1","first-page":"3996","article-title":"Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes","volume":"49","author":"Takigawa M","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.08.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2007.06.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700249"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200402106"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2009.10.008"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac026117i"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400417"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008208209160269"},{"key":"e_1_2_8_25_1","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation","volume":"29","author":"Armbruster DA","year":"2008","journal-title":"Clin Biochem Rev"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.20.11715"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2012.01.006"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0697-174"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.15.10738"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400934"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/359843a0"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.9.4604"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orthres.2004.03.002"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209129"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.934301"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1205"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M607135200"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.10.008"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050738"},{"key":"e_1_2_8_40_1","first-page":"3524","article-title":"558 The involvement of hypoxia\u2010inducible transcription factor\u20101\u2010dependent pathway in nickel carcinogenesis","volume":"63","author":"Salnikow K","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-010-9342-y"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5294"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.07.003"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.51.32529"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.10.017"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652413804486214"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24547"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310124200"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.329771"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608289200"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.392"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00073.2014"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.170"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2013.10.012"},{"key":"e_1_2_8_55_1","first-page":"19331","article-title":"FOXO3a regulates oxygen\u2010responsive expression of tumor necrosis factor receptor 2 in human dermal microvascular endothelial cells","volume":"284","author":"Ding B","year":"2009","journal-title":"J Cell Biol"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr901209s"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.26394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.26394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.26394","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.26394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T14:33:09Z","timestamp":1753799589000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.26394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,18]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/jcb.26394"],"URL":"https:\/\/doi.org\/10.1002\/jcb.26394","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,18]]}}}