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MMP7 is a member of the matrix metalloproteinase family that plays an important role in cell invasion. Here we evaluate the possible role and underlying mechanism of MMP7 in the leptin\u2010mediated cell invasion in ovarian cancer cell lines. All experiments were carried out in cultured SKOV3, OVCAR3, and CaoV\u20103 ovarian cell lines. MMP7 expression was determined using the Western blot following treatment to various concentrations of leptin for defined time intervals. The activation of ERK, JNK, and P38 MAP kinases were determined using Western blotting. Wound healing and BD matrigel invasion assays were used to measure cell migration and invasion. The siRNA approach and pharmacological inhibitors of ERK and JNK pathway were used to confirm the receptor\u2010dependent effect of leptin and a role for ERK and JNK pathway. Zymography assay was employed to determine MMP2 and MMP9 activation. Results show that leptin induces ERK1\/2 and JNK1\/2 activation and subsequently promotes MMP7 expression in SKOV3 (4.8\u2009\u00b1\u20090.14 fold of control, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) and OVCAR3 (3.1\u2009\u00b1\u20090.19 fold of control, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) ovarian cancer cell lines. These effects was reversed by knockdown of OB\u2010Rb and\/or pre\u2010incubation with PD98059 (ERK1\/2 inhibitor), SP600125 (JNK1\/2 inhibitor). Gelatin zymography showed that MMP7 gene silencing attenuated leptin\u2010induced MMP9 activation in SKOV3 cell line. Taken together, our results suggest new evidences for a modulatory effect of leptin in regulation of ovarian cancer cell invasion by stimulating MMP7 expression via ERK and JNK pathways.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26396","type":"journal-article","created":{"date-parts":[[2017,9,8]],"date-time":"2017-09-08T08:46:40Z","timestamp":1504860400000},"page":"2333-2344","source":"Crossref","is-referenced-by-count":47,"title":["RETRACTED: Leptin induces matrix metalloproteinase 7 expression to promote ovarian cancer cell invasion by activating ERK and JNK pathways"],"prefix":"10.1002","volume":"119","author":[{"given":"Ahmad","family":"Ghasemi","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Bioinformatics Research Center School of Pharmacy and Pharmaceutical Sciences Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Isaac","family":"Hashemy","sequence":"additional","affiliation":[{"name":"Surgical Oncology Research Center Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1775-8761","authenticated-orcid":false,"given":"Mahmoud","family":"Aghaei","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Bioinformatics Research Center School of Pharmacy and Pharmaceutical Sciences Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3194-3573","authenticated-orcid":false,"given":"Mojtaba","family":"Panjehpour","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Bioinformatics Research Center School of Pharmacy and Pharmaceutical Sciences Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.02.008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0297"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgq276"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051578"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-008-9231-x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomed.2013.09.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgt028"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.01169"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-8-74"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","unstructured":"KatoS Abarz\u00faa Catal\u00e1nL DelpianoC et al. 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