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Resistance to conventional treatments and chemotherapeutics is a typical feature of PDAC. To investigate the causes of drug resistance it is essential to deeply investigate the mechanism of action of chemotherapeutics. In this study, we performed an in depth shotgun proteomic approach using the label\u2010free proteomic SWATH\u2010MS analysis to investigate novel insights of the mechanism of action of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) in PDAC cells. This proteomic analysis in PaCa44 cells and data elaboration of TSA\u2010regulated proteins by bioinformatics showed an overall up\u2010regulation of cytokeratins and other proteins related to the cytoskeleton organization, keratinization, and apoptotic cell death. On the contrary, a large amount of the down\u2010regulated proteins by TSA treatment belongs to the cellular energetic metabolism and to the machinery of protein synthesis, such as ribosomal proteins, determining synergistic cell growth inhibition by the combined treatment of TSA and the glycolytic inhibitor 2\u2010deoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose in a panel of PDAC cell lines. Data are available via ProteomeXchange with identifier PXD007801.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26436","type":"journal-article","created":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T15:12:12Z","timestamp":1509635532000},"page":"2696-2707","source":"Crossref","is-referenced-by-count":34,"title":["Trichostatin A alters cytoskeleton and energy metabolism of pancreatic adenocarcinoma cells: An in depth proteomic study"],"prefix":"10.1002","volume":"119","author":[{"given":"Elisa","family":"Dalla Pozza","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Biomedicine and Movement University of Verona Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Manfredi","sequence":"additional","affiliation":[{"name":"Department of Sciences and Technological Innovation University of Piemonte Orientale Alessandria Italy"},{"name":"ISALIT S.r.l. 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