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Evasion mechanisms are the main characteristic of these tumor cells to escape of immune response. Extracellular nucleotides and nucleosides play an important role in inflammatory and immune responses. In this study, we analyzed the expression and activity of purinergic system enzymes in platelets and lymphocytes, ATP levels quantification, as well the level of pro and anti\u2010inflammatory interleukins in the serum of 23 patients with surgical melanoma removal (CM group) and 23 control subjects (CT group). Results showed a decrease in ATP, ADP, and AMP hydrolysis and an increase in ATP levels quantification in CM group. The pro\u2010inflammatory cytokines were elevated in CM group when compared to CT group. These results suggest an inflammatory process, even after surgical removal, due to elevated extracellular ATP levels. Besides, CM group displayed an increase in IL\u201010 levels and an increased in ADA activity in platelets and lymphocytes. Once adenosine and IL\u201010 are anti\u2010inflammatory molecules, these results indicate a down\u2010regulation of immune system front to malignant process. The alteration in nucleotide and nucleoside hydrolysis reinforces the purinergic systems role in this cancer. Therefore, even after surgical removal, the purinergic system can develop a chronic inflammatory micro\u2010environment that can influence directly on relapse or metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26551","type":"journal-article","created":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T14:32:39Z","timestamp":1513002759000},"page":"3980-3988","source":"Crossref","is-referenced-by-count":23,"title":["High levels of extracellular ATP lead to chronic inflammatory response in melanoma patients"],"prefix":"10.1002","volume":"119","author":[{"given":"Aline","family":"Manica","sequence":"first","affiliation":[{"name":"Campus Santa Maria Federal University of Santa Maria Santa Maria RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexsandra Martins","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Campus Chapec\u00f3 Federal University of Fronteira Sul Chapec\u00f3 Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreia Machado","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Campus Chapec\u00f3 Federal University of Fronteira Sul Chapec\u00f3 Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Moreno","sequence":"additional","affiliation":[{"name":"Campus Chapec\u00f3 Community University of Chapec\u00f3 Region Chapec\u00f3 Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Bittencourt","family":"Leal","sequence":"additional","affiliation":[{"name":"Campus Santa Maria Federal University of Santa Maria Santa Maria RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anielen","family":"Dutra Da Silva","sequence":"additional","affiliation":[{"name":"Campus Santa Maria Federal University of Santa Maria Santa Maria RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosa Chitolina","family":"Schetinger","sequence":"additional","affiliation":[{"name":"Campus Santa Maria Federal University of Santa Maria Santa Maria RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera Maria Melchiors","family":"Morsch","sequence":"additional","affiliation":[{"name":"Campus Santa Maria Federal University of Santa Maria Santa Maria RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9263-4980","authenticated-orcid":false,"given":"Margarete Dulce","family":"Bagatini","sequence":"additional","affiliation":[{"name":"Campus Chapec\u00f3 Federal University of Fronteira Sul Chapec\u00f3 Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0133363"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-1600"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2015.04.014"},{"key":"e_1_2_8_6_1","first-page":"177","article-title":"Purinergic regulation of the immune system","volume":"3","author":"Cekic C","year":"2016","journal-title":"Nat Rev"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.280.1.C81"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.04.013"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385526-8.00010-2"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200939741"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002680"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031205"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2004.11.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2011.09.003"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2011.09.007"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-014-9405-8"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-197011000-00017"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537109609023582"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(03)00178-6"},{"key":"e_1_2_8_20_1","first-page":"77","article-title":"Isolation of mononuclear cells and granulocytes from human blood. 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