{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:24:53Z","timestamp":1784334293271,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T00:00:00Z","timestamp":1513036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T00:00:00Z","timestamp":1543536000000},"content-version":"vor","delay-in-days":353,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T00:00:00Z","timestamp":1513036800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study aims to investigate the regulative role of microRNA\u201093 (miR\u201093) in mouse cardiac microvascular endothelial cells (CMECs) injury and inflammatory response by negatively targeting SPP1 gene via the NF\u2010\u03baB signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Healthy Balb\/c mice were recruited to establish a mouse model with myocarditis using the CVB3 virus. Mice were grouped into normal, blank, negative control (NC), miR\u201093 inhibitor, miR\u201093 mimic, SPP1 short hairpin RNA (shRNA), and miR\u201093 mimic+SPP1 shRNA groups. Reverse transcription quantitative polymerase chain reaction and Western blot analysis were applied to determine the expressions of miR\u201093, SPP1, VEGFA, p50, p65, Bax, and Bcl\u20102. MTT assay was conducted to evaluate cell viability, annexin V\u2010fluorescein isothiocyanate\/propidium iodide double staining was conducted to examine cell apoptosis, enzyme\u2010linked immunosorbent assay was conducted to measure secretion of inflammatory factors, and chemical colorimetry was conducted to determine NO secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SPP1 was a target gene of miR\u201093. Compared with the normal group, other six groups showed increased expressions of SPP1, p50, p65, VEGFA, and Bax, as well as cell apoptosis rate and secretion of cell inflammatory factors, and decreased expression of Bcl\u20102, cell viability, and NO secretion. Compared with the blank group, the miR\u201093 inhibitor group showed elevated expressions of SPP1, p50, p65, VEGFA, and Bax, as well as cell apoptosis rate and secretion of cell inflammatory factors, and reduced Bcl\u20102, cell viability, and NO secretion. While the miR\u201093 mimic and SPP1 shRNA groups displayed opposite results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taking our results together, we conclude that upregulation of miR\u201093 reduces CMECs injury and inflammatory response by negatively targeting SPP1 via inactivating the NF\u2010\u03baB signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26567","type":"journal-article","created":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T13:28:58Z","timestamp":1513085338000},"page":"2847-2858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>Retracted<\/i>: Effects of microrna\u201093 on mouse cardiac microvascular endothelial cells injury and inflammatory response by mediating SPP1 through the NF\u2010\u039aB pathway"],"prefix":"10.1002","volume":"120","author":[{"given":"Su\u2010Xia","family":"Ma","sequence":"first","affiliation":[{"name":"Second 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