{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:38:02Z","timestamp":1780450682773,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute for Research in Reproductive Health (Mumbai, India)","award":["NIRRH\/RA\/543\/09-2017"],"award-info":[{"award-number":["NIRRH\/RA\/543\/09-2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>MicroRNAs (miRNAs) mediate post\u2010transcriptional gene suppression and are a critical component of the complex regulatory networks in epithelial immune responses. Transcription of miRNA genes in epithelial cells can be elaborately controlled through Toll\u2010like receptors (TLRs), and associated nuclear factor kappa\u2010light\u2010chain\u2010enhancer of activated B cells (NF\u2010\u03baB) and mitogen\u2010activated protein kinase (MAPK) pathways, leading to nuclear transcription factor associated\u2010transactivation and transrepression of miRNAs. MiRNA, let\u20107f is involved in the regulation of innate immune responses post TLR3 stimulation in human endocervical cells (End1\/E6E7) and decreased let\u20107f is associated with poor immune activation. Thus, expression of let\u20107f is under strict control. However, the mechanism by which let\u20107f is regulated in these cells is not known. Therefore, in the present study, we have investigated the role of MAPK and NF\u2010\u03baB in the transcription of let\u20107f. We report that signalling of TLR3, results in activation of multiple signalling pathways including MAPK\/ERK, JNK, p38, and NF\u2010\u03baB. Of these MAPK\/ p38 and JNK directly influence the expression of let\u20107f in End1\/E6E7 cells. Inhibition of ERK and NF\u2010\u03baB up regulates the expression of let\u20107f and its transcription factor, C\/EBP\u03b2. In conclusion, we have identified a system through which TLR3 mediated immune response is regulated by C\/EBP\u03b2 and let\u20107f through the temporal activation of MAPK and NF\u2010\u03baB in human endocervical cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26665","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T16:55:00Z","timestamp":1515689700000},"page":"4751-4759","source":"Crossref","is-referenced-by-count":14,"title":["MAPK and NF\u2010\u03baB signalling pathways regulate the expression of miRNA, let\u20107f in human endocervical epithelial cells"],"prefix":"10.1002","volume":"119","author":[{"given":"Kanchana K.","family":"Ayyar","sequence":"first","affiliation":[{"name":"Division of Molecular Immunology and Microbiology Indian Council of Medical Research\u2010National Institute for Research in Reproductive Health (ICMR\u2010NIRRH) Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0735-7662","authenticated-orcid":false,"given":"Kudumula V. R.","family":"Reddy","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology and Microbiology Indian Council of Medical Research\u2010National Institute for Research in Reproductive Health (ICMR\u2010NIRRH) Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.04.040"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12165"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2035"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00031-10"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3495"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90403-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000757"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.11.011"},{"key":"e_1_2_9_11_1","first-page":"116","article-title":"MicroRNAs: the Key Regulators of PRR 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