{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T04:28:00Z","timestamp":1770956880759,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002958","name":"Drug Applied Research Center, Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["94\/47"],"award-info":[{"award-number":["94\/47"]}],"id":[{"id":"10.13039\/501100002958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Chemotherapy is the most common method to treat leukemia as well as other types of human cancers. However, drug resistance has remained as the main challenge against the efficacy of treatments. Furthermore, having various adverse effects, chemotherapy drugs are becoming replaced by natural modalities for cancer therapy. In this regard, herbal components such as resveratrol and prednisolone have been identified to sensitize the leukemic cells to programmed cell death through a set of complex processes. In this study, we have examined DNA methylation on the human multidrug resistance gene 1 (<jats:italic>MDR1<\/jats:italic>) as a well\u2010known marker for cellular drug resistance. We evaluated the effect of resveratrol and prednisolone on DNA methylation patterns of <jats:italic>MDR1<\/jats:italic> gene promoter in the CCRF\u2010CEM cell line as a representative for acute lymphoblastic leukemia. The study was aimed to clarify whether the <jats:italic>MDR1<\/jats:italic> gene expression is regulated via DNA promoter methylation as a potential underlying mechanism, following exposure to resveratrol and prednisolone. Our data revealed that despite a strong influence to down\u2010regulate the <jats:italic>MDR1<\/jats:italic> expression, Resveratrol and Prednisolone did not alter the methylation pattern, suggesting other regulatory mechanisms in controlling the <jats:italic>MDR1<\/jats:italic> expression in CCRF\u2010CEM cell line. Unchanged status of DNA methylation of <jats:italic>MDR1<\/jats:italic> gene may suggest that Resveratrol and Prednisolone causes the gene expression changes through a distinct mechanism which requires further studies to be understood. A more detailed understanding of the mechanisms beyond the regulation of the genes involved in cancer formation will help to design novel therapeutic strategies to fight the human cancers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26709","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T19:34:03Z","timestamp":1517254443000},"page":"4890-4896","source":"Crossref","is-referenced-by-count":17,"title":["Regulatory effect of resveratrol and prednisolone on <i>MDR1<\/i> gene expression in acute lymphoblastic leukemia cell line (CCRF\u2010CEM): An epigenetic perspective"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2340-6594","authenticated-orcid":false,"given":"Milad","family":"Zadi Heydarabad","sequence":"first","affiliation":[{"name":"Drug Applied Research Center Tabriz University of Medical Sciences Tabriz 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