{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:15:11Z","timestamp":1781198111948,"version":"3.54.1"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T00:00:00Z","timestamp":1539820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Visceral leishmaniasis (VL) is caused by the parasites of <jats:italic>Leishmania donovani<\/jats:italic> complex, leads to the death of 20\u2009000 to 40\u2009000 people from 56 affected countries, worldwide. Till date, there is not a single available vaccine candidate to prevent the VL infection, and treatment only relies upon expensive and toxic chemotherapeutic options. Consequently, immunoinformatics approach was applied to design a multiepitope\u2010based subunit vaccine to enhance the humoral as well as cell\u2010mediated immunity. Constructed vaccine candidate was further subjected to evaluation on allergenicity and antigenicity and physiochemical parameters. Later on, disulfide engineering was performed to increase the stability of vaccine construct. Also, molecular docking and molecular dynamics simulation study were performed to check the binding affinity and stability of toll\u2010like receptor\u20104 to vaccine construct complex. Finally, codon optimization and in silico cloning were performed to ensure the expression of proposed vaccine construct in a microbial expression system.<\/jats:p>","DOI":"10.1002\/jcb.26719","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T17:12:57Z","timestamp":1517245977000},"page":"1141-1155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Exploring sand fly salivary proteins to design multiepitope subunit vaccine to fight against visceral leishmaniasis"],"prefix":"10.1002","volume":"120","author":[{"given":"Rajan Kumar","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, Central University of Rajasthan Ajmer India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-0596","authenticated-orcid":false,"given":"Vijay 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