{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:19:18Z","timestamp":1778642358096,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T00:00:00Z","timestamp":1525219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Interleukin\u201010 (IL\u201010), produced generally by monocyte, T helper type 2 (Th2), and regulatory T cells (Treg), plays a central role in controlling inflammatory responses and regulating the immune response of the IL\u201010 mRNA expression. It is significantly down\u2010regulated in many autoimmune diseases such as Beh\u00e7et's disease; this is mostly associated with more aggressive complications. Nevertheless, the essential molecular process for its low expression has not been completely realized. The aim of this project was attempted to estimate the gene expression, promoter methylation, and protein levels to IL\u201010's down\u2010regulated expression. In this study, blood samples from 51 (4 missed) patients and 63 (2 missed) healthy controls were taken, with the mononuclear cells isolated by the Ficoll Protocol. DNA and RNA were then subsequently extracted. Promoter methylation levels were evaluated by MeDIP\u2010qPCR. Following this, the extracted RNA was converted to cDNA using the RT\u2010PCR method, with the expression of IL\u201010 later evaluated by Real\u2010time PCR. And then, serum levels of IL\u201010 were measured using ELISA method. As we expected, the expression level of the IL\u201010 gene was seen to significantly decline in the patient group in comparison to the control. Also, the rate of promoter methylation was significantly higher in the IL\u201010 mRNA low expression group (patient group) compared to its high expression group (healthy group) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). We revealed that hypermethylation of promoter region was the principal defect for the IL\u201010 mRNA low expression in patients with Beh\u00e7et's disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26809","type":"journal-article","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T17:09:01Z","timestamp":1525280941000},"page":"6614-6622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Hypermethylation of IL\u201010 gene is responsible for its low mRNA expression in Beh\u00e7et's disease"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2202-9598","authenticated-orcid":false,"given":"Shahriar","family":"Alipour","sequence":"first","affiliation":[{"name":"Molecular Medicine Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Connective Tissue Disease Research Center Tabriz University of Medical Science Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5847-3594","authenticated-orcid":false,"given":"Mohammad","family":"Nouri","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Khabbazi","sequence":"additional","affiliation":[{"name":"Connective Tissue Disease Research Center Tabriz University of Medical Science Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasser","family":"Samadi","sequence":"additional","affiliation":[{"name":"Cancer Biochemistry Cancer Biotechnology Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohreh","family":"Babaloo","sequence":"additional","affiliation":[{"name":"Department of Immunology Medicine Faculty Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somayeh","family":"Abolhasani","sequence":"additional","affiliation":[{"name":"MSc of Biostatistics Faculty of Dentistry Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jafar","family":"Farhadi","sequence":"additional","affiliation":[{"name":"Molecular Medicine Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Connective Tissue Disease Research Center Tabriz University of Medical Science Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neda","family":"Roshanravan","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Center Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Students Research Committee Nutrition Research Center School of Nutrition Tabriz University of Medical Science Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Golamreza","family":"Jadideslam","sequence":"additional","affiliation":[{"name":"Molecular Medicine Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences Tabriz Iran"},{"name":"Connective Tissue Disease Research Center Tabriz University of Medical Science Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebrahim","family":"Sakhinia","sequence":"additional","affiliation":[{"name":"Department of Genetics Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar757"},{"key":"e_1_2_7_3_1","first-page":"35","volume-title":"Epidemiology of Beh\u00e7et's syndrome and regional differences in disease expression. 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